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Volume 27, Number 6—June 2021

Cutaneous Leishmaniasis Caused by an Unknown Leishmania Strain, Arizona, USA

Marcos de AlmeidaComments to Author , Yueli Zheng, Fernanda S. Nascimento, Henry Bishop, Vitaliano A. Cama, Dhwani Batra, Yvette Unoarumhi, Abaseen K. Afghan, Vivian Y. Shi1, Philip E. LeBoit, Eugene W. Liu, and Fariba M. Donovan
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M. de Almeida, Y. Zheng, F.S. Nascimento, H. Bishop, V.A. Cama, D. Batra, Y. Unoarumhi, E.W. Liu); Eagle Global Scientific, San Antonio, Texas, USA (Y. Zheng); University of Arizona College of Medicine, Tucson, Arizona, USA (A.K. Afghan, V.Y. Shi, F.M. Donovan); University of California, San Francisco, California, USA (P.E. LeBoit)

Main Article


Comparative analysis of sequences of 13 genes showing percent sequence identity between a Leishmania isolate from a patient in Arizona, USA, and other Leishmania species*

Gene ORF† Arizona isolate vs. L. (L.) donovani
Arizona isolate vs. L. (L.) infantum
Arizona isolate vs. L. (L.) mexicana
Arizona isolate vs. L. (M.) enrietti
Arizona isolate vs. L. (M.) martiniquensis
MiSeq fragment length, bp†
Accession no. ID, % Accession no. ID, % Accession no. ID, % Accession no. ID, % Accession no. ID, %
ACT AY079087 97.26 GQ246778 96.86 GQ246777 97.29 ATAF02000030 88.95 NA 1,145

*Accession numbers are for GenBank. ID, % indicates percent identity between sequences. ACT, β-actin; ASAT, aspartate aminotransferase; GAPDH, glyceraldeyde-3-phosphate dehydrogenase; GPI, glucose-6-phosphate isomerase; G6PDH, glucose-6-phosphate dehydrogenase; HSP70, heat-shock protein 70; ICD, isocitrate dehydrogenase; ID, identity; ITS, internal transcribed spacer; ME, malic enzyme; MPI, mannose-6-phosphate isomerase; NA, not available; NADP, nicotinamide adenine dinucleotide phosphate; ORF, open reading frame; PGD, PGM, 6-phosphogluconate dehydrogenase; PGM, phosphoglucomutase; –, not applicable.
†GenBank accession numbers for the Arizona isolate were MT786532 (ACT), MT786533 (ASAT), MT786534 (G6PDH), MT786535 (GAPDH), MT786536 (GPI), MT786537 (ICD), MT786538 (ME), MT786539 (MPI), MT786540 (PGD), MT786541 (PGM), MT813027 (HSP70), MT776522 (18S rRNA) and ITS-1 rRNA region MT791386 (rRNA-ITS region). Sequences for ME and HSP70 were shorter by 206 and 20–30 bp, respectively, when compared with sequences in GenBank.
‡Only a 37-bp partial sequence of ITS rRNA of L. (M.) martiniquensis is available for comparison.

Main Article

1Current affiliation: University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.

Page created: March 09, 2021
Page updated: May 18, 2021
Page reviewed: May 18, 2021
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