Volume 27, Number 6—June 2021
Research Letter
Rapid Antigen Test for Postmortem Evaluation of SARS-CoV-2 Carriage
Table
Patient characteristics and postmortem data for investigation of rapid antigen test for postmortem evaluation of SARS-CoV-2 carriage, Graz, Austria*
Characteristic | RAT cohort, n = 30 | Culture cohort, n = 11 |
---|---|---|
Age, y, median (range) |
78 (62–93) |
79 (65–93) |
Sex, no. (%) | ||
M | 14 (47.7) | 6 (56) |
F |
16 (53.3) |
5 (45.4) |
Disease duration,† d, median (range) | 12 (1–43) | 9 (3–34) |
Postmortem interval‡, h, median (range) |
22 (8–124) |
25 (14–68) |
qRT-PCR positive, no. (%) |
24 (80) |
11 (100) |
Ct value, median (range) | ||
E gene | 22.8 (14.1–37.3) | 19.9 (13.7–36.0) |
N gene |
26.9 (18.0–34.6) |
24.6 (17.3–33.7) |
Cultivation positive, no. (%) |
NA |
7 (63.6) |
RAT positive, no. (%) |
17 (56.7%) |
NA |
Total RAT specificity (95% CI§), n = 30 |
100% (61%–100%) |
NA |
RAT sensitivity (95% CI§), n = 30 | 70.8% (50.8%–85.1%) | NA |
Total, n = 30 | ||
Ct <35,¶ n = 23 | 73.9% (53.5%–87.5%) | NA |
Ct <30,¶ n = 18 | 94.4% (74.2%–99.7%) | NA |
Ct <25,¶ n = 16 | 100% (80.6%–100%) | NA |
*Ct, cycle threshold; E, envelope; N, nucleocapsid; NA, not applicable; qRT-PCR, quantitative reverse transcription PCR: RAT, rapid antigen test; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. †Interval from first positive (antemortem) SARS-CoV-2 PCR to death. ‡Interval from death to specimen sampling. §Determined via the hybrid Wilson/Brown method (10). ¶Determined via E gene qRT-PCR.
References
- Centers for Disease Control and Prevention. Interim guidance for antigen testing for SARS-CoV-2 [cited 2021 Mar 27]. https://www.cdc.gov/coronavirus/2019-ncov/lab/resources/antigen-tests-guidelines.html
- Dinnes J, Deeks JJ, Berhane S, Taylor M, Adriano A, Davenport C, et al.; Cochrane COVID-19 Diagnostic Test Accuracy Group. Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection. Cochrane Database Syst Rev. 2021;3:
CD013705 .PubMedGoogle Scholar - Albert E, Torres I, Bueno F, Huntley D, Molla E, Fernández-Fuentes MÁ, et al. Field evaluation of a rapid antigen test (Panbio™ COVID-19 Ag Rapid Test Device) for COVID-19 diagnosis in primary healthcare centres. Clin Microbiol Infect. 2021;27:472.e7–10. DOIPubMedGoogle Scholar
- Iglὁi Z, Velzing J, van Beek J, van de Vijver D, Aron G, Ensing R, et al. Clinical evaluation of Roche SD Biosensor rapid antigen test for SARS-CoV-2 in municipal health service testing site, the Netherlands. Emerg Infect Dis. 2021;27. DOIPubMedGoogle Scholar
- Centers for Disease Control and Prevention. Collection and submission of postmortem specimens from deceased persons with confirmed or suspected COVID-19: postmortem guidance [cited 2021 Mar 27]. https://www.cdc.gov/coronavirus/2019-ncov/hcp/guidance-postmortem-specimens.html
- Singanayagam A, Patel M, Charlett A, Lopez Bernal J, Saliba V, Ellis J, et al. Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020. Euro Surveill. 2020;25:
2001483 . DOIPubMedGoogle Scholar - Puelles VG, Lütgehetmann M, Lindenmeyer MT, Sperhake JP, Wong MN, Allweiss L, et al. Multiorgan and renal tropism of SARS-CoV-2. N Engl J Med. 2020;383:590–2. DOIPubMedGoogle Scholar
- Jefferson T, Spencer EA, Brassey J, Heneghan C. Viral cultures for COVID-19 infectious potential assessment—a systematic review. Clin Infect Dis. 2020 Dec 20 [Epub ahead of print]. DOIGoogle Scholar
- Loibner M, Langner C, Regitnig P, Gorkiewicz G, Zatloukal K. Biosafety requirements for autopsies of patients with COVID-19: example of a BSL-3 autopsy facility designed for highly pathogenic agents. Pathobiology. 2021;88:37–45. DOIPubMedGoogle Scholar