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Volume 27, Number 7—July 2021
Dispatch

Prevalence of Middle East Respiratory Syndrome Coronavirus in Dromedary Camels, Tunisia

Simone Eckstein, Rosina Ehmann, Abderraouf Gritli, Houcine Ben Yahia, Manuel Diehl, Roman Wölfel, Mohamed Ben Rhaiem, Kilian Stoecker1Comments to Author , Susann Handrick1, and Mohamed Ben Moussa1
Author affiliations: Bundeswehr Institute of Microbiology, Munich, Germany (S. Eckstein, R. Ehmann, M. Diehl, R. Wölfel, K. Stoecker, S. Handrick); Ministry of National Defense, General Directorate of Military Health, Veterinary Service, Tunis, Tunisia (A. Gritli, H. Ben Yahia, M. Ben Rhaiem); Military Hospital of Instruction of Tunis Department of Virology, Tunis (M. Ben Moussa)

Main Article

Figure 2

Phylogenetic analysis of MERS-CoV samples from dromedary camels in Tunisia, conducted by using the spike RBD. We used 720-bp fragments of the MERS-CoV spike RBD amplified from nasal swab samples of 13 dromedary camels and published RBD sequences of representative MERS-CoV strains from other countries to create the phylogenetic tree using Geneious Prime Tree Builder (Geneious Biologics, https://www.geneious.com). Branches are shaded by country: red represents sequences from Tunisia (this study); brown represents Morocco, pink Burkina Faso, dark green Nigeria, blue Egypt, dark blue Qatar, green Saudi Arabia, yellow South Korea, purple United Arab Emirates, and orange Oman. GenBank accession numbers are provided for reference sequences. Numbers indicate bootstrap values (1,000 pseudo-replicates). Scale bar indicates sequence divergence (% nucleotide substitutions). MERS-CoV, Middle East respiratory syndrome coronavirus; RBD, receptor-binding protein.

Figure 2. Phylogenetic analysis of MERS-CoV samples from dromedary camels in Tunisia, conducted by using the spike RBD. We used 720-bp fragments of the MERS-CoV spike RBD amplified from nasal swab samples of 13 dromedary camels and published RBD sequences of representative MERS-CoV strains from other countries to create the phylogenetic tree using Geneious Prime Tree Builder (Geneious Biologics, https://www.geneious.com). Branches are shaded by country: red represents sequences from Tunisia (this study); brown represents Morocco, pink Burkina Faso, dark green Nigeria, blue Egypt, dark blue Qatar, green Saudi Arabia, yellow South Korea, purple United Arab Emirates, and orange Oman. GenBank accession numbers are provided for reference sequences. Numbers indicate bootstrap values (1,000 pseudo-replicates). Scale bar indicates sequence divergence (% nucleotide substitutions). MERS-CoV, Middle East respiratory syndrome coronavirus; RBD, receptor-binding protein.

Main Article

1These authors contributed equally to this article.

Page created: May 06, 2021
Page updated: June 17, 2021
Page reviewed: June 17, 2021
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