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Volume 27, Number 7—July 2021
Research Letter

Possible Human-to-Dog Transmission of SARS-CoV-2, Italy, 2020

Nicola DecaroComments to Author , Gabriele Vaccari, Alessio Lorusso, Eleonora Lorusso, Luca De Sabato, Edward I. Patterson, Ilaria Di Bartolo, Grant L. Hughes, Liana Teodori, Costantina Desario, Barbara Colitti, Dominga Ricci, Domenico Buonavoglia, Sergio Rosati, Vito Martella, Cesare Cammà, Umberto Agrimi, and Gabriella Elia
Author affiliations: University of Bari, Valenzano, Italy (N. Decaro, E. Lorusso, C. Desario, D. Buonavoglia, V. Martella, G. Elia); Istituto Superiore di Sanità, Rome, Italy (G. Vaccari, L. De Sabato, I. Di Bartolo, U. Agrimi); Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale,” Teramo, Italy (A. Lorusso, L. Teodori, C. Cammà); Liverpool School of Tropical Medicine, Liverpool, UK (E.I. Patterson, G.L. Hughes); University of Turin, Turin, Italy (B. Colitti, S. Rosati); Ambulatorio Veterinario Dott.ssa Ricci Dominga, Andria, Italy (D. Ricci)

Main Article

Table

Molecular and serologic testing of dog with severe acute respiratory syndrome coronavirus 2 infection, Italy, 2020*

Date of sample collection Real-time reverse transcription PCR Ct values
 Serologic assay results
Oral Nasal Rectal ELISA ID.vet† ELISA In3Diagnostic‡ PRNT80§ VNT¶
2020 Nov 4 35.7** 35.7** ND ND ND ND ND
2020 Nov 6 ND 37.64 ND ND ND ND ND
2020 Nov 7 35.61 – ND ND ND ND ND
2020 Nov 8 ND – 40.71 ND ND ND ND
2020 Nov 9 ND – 36.04 ND ND ND ND
2020 Nov 10 – – – ND ND ND ND
2020 Nov 11 – – ND ND ND ND ND
2020 Nov 12 – – ND ND ND ND ND
2020 Nov 13 – – ND ND ND ND ND
2020 Nov 14 – – ND ND ND ND ND
2020 Nov 15 – – ND ND ND ND ND
2020 Nov 27 ND ND ND – + (23%)†† 1:80 1:10
2020 Dec 12 ND ND ND – – 1:80 1:20

*Ct, cycle threshold; ND, not done; PRNT80, 80% plaque reduction neutralization test; VNT, virus neutralization test; –, negative; +, positive.
†ID Screen SARS-CoV-2 Double Antigen Multi-species ELISA (ID.vet, https://www.id-vet.com).
‡Eradikit COVID19-Multispecies (In3Diagnostic, http://www.in3diagnostic.com).
§Antibody titer expressed as the highest serum dilution with 80% reduction in plaques in inoculated VERO-E6 cells compared with the control.1:20 was the lowest serum dilution tested.
¶Antibody titer expressed as the highest serum dilution preventing the appearance of cytopathic effect in inoculated VERO-E6 cells. 1:10 was the lowest serum dilution tested.
**Pooled oral and nasal swab specimens.
††Ratio between the optical densities of the tested serum and the positive control (cutoff value = 20%).

Main Article

Page created: March 23, 2021
Page updated: June 17, 2021
Page reviewed: June 17, 2021
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