Continued Circulation of Tick-Borne Encephalitis Virus Variants and Detection of Novel Transmission Foci, the Netherlands
Helen J. Esser
, Stephanie M. Lim, Ankje de Vries, Hein Sprong, Dinant J. Dekker, Emily L. Pascoe, Julian W. Bakker, Vanessa Suin, Eelco Franz, Byron E.E. Martina, and Constantianus J.M. Koenraadt
Author affiliations: Wageningen University and Research, Wageningen, the Netherlands (H.J. Esser, D.J. Dekker, E.L. Pascoe, J.W. Bakker, C.J.M. Koenraadt); Artemis One Health Research Institute, Delft, the Netherlands (S.M. Lim, B.E.E. Martina); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (A. de Vries, H. Sprong, E. Franz); Sciensano, Brussels, Belgium (V. Suin)
Figure 2. Maximum-likelihood phylogenetic tree of polyprotein sequences obtained from tick-borne encephalitis virus RNA–positive Ixodes ricinus ticks collected from 3 locations in the Netherlands during 2016–2020 (in bold). Additional published sequences obtained from GenBank are included for reference. Louping ill virus is used as the outgroup. Sample ID or GenBank accession numbers are indicated for each sequence, with location in brackets (if known) and country code, original isolation source, and collection year of each sample. Numbers next to each branch indicate the percentage of trees resulting from bootstrapping on the basis of 1,000 pseudoreplicate datasets for which the associated taxa clustered together. Scale bar represents the percentage of genetic variation along tree branches.
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