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Volume 28, Number 4—April 2022
Research

Increased Attack Rates and Decreased Incubation Periods in Raccoons with Chronic Wasting Disease Passaged through Meadow Voles

S. Jo Moore, Christina M. Carlson, Jay R. Schneider, Christopher J. Johnson, and Justin J. GreenleeComments to Author 
Author affiliations: US Department of Agriculture, Ames, Iowa, USA (S.J. Moore, J.J. Greenlee); US Geological Survey National Wildlife Health Center, Madison, Wisconsin, USA (C.M. Carlson, J.R. Schneider, C.J. Johnson).

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Figure 4

Patterns of histopathology and immunohistopathology in brains from 2 raccoons inoculated with the agent of chronic wasting disease (CWD). Panels A, C, E, and G show results for raccoon 2, inoculated with the agent of CWD from white-tailed deer; panels B, D, F, and H) show results for raccoon 9, inoculated with CWD from a vole that had been inoculated with the 4th vole-passage of the agent of CWD from white-tailed deer. All images original magnification ×20. A–D) Medulla at the level of the obex. A) Raccoon 2 shows no spongiform change in the dorsal motor nucleus of the vagus nerve (DMNV) (above dashed line) or hypoglossal nucleus (below dashed line). Hematoxylin and eosin (H&E) stain. B) Raccoon 9 shows mild to moderate spongiform change in the DMNV. H&E stain. C) Raccoon 2 shows very mild PrPSc immunoreactivity in the DMNV and no immunoreactivity in neurons of the hypoglossal nucleus. PrP antibodies F89/160.1.5 and F99/97.6.1. D) Raccoon 9 shows moderate PrPSc immunoreactivity in the neuropil of the DMNV and marked intraneuronal immunoreactivity in the hypoglossal nucleus. PrP antibodies F89/160.1.5 and F99/97.6.1. E–H) Caudate nucleus. E) Raccoon 2 shows moderate diffuse spongiform change. H&E stain. F) Raccoon 9 shows marked diffuse spongiform change. H&E stain. G) Raccoon 2 shows diffuse neuropil PrPSc immunoreactivity and prominent extracellular PrPSc accumulation on neurons (arrowheads). PrP antibodies F89/160.1.5 and F99/97.6.1. H) Raccoon 9 shows marked intracellular PrPSc immunoreactivity in neurons (arrowheads) and glial cells (arrows). PrP antibodies F89/160.1.5 and F99/97.6.1.

Figure 4. Patterns of histopathology and immunohistopathology in brains from 2 raccoons inoculated with the agent of chronic wasting disease (CWD). Panels A, C, E, and G show results for raccoon 2, inoculated with the agent of CWD from white-tailed deer; panels B, D, F, and H) show results for raccoon 9, inoculated with CWD from a vole that had been inoculated with the 4th vole-passage of the agent of CWD from white-tailed deer. All images original magnification ×20. A–D) Medulla at the level of the obex. A) Raccoon 2 shows no spongiform change in the dorsal motor nucleus of the vagus nerve (DMNV) (above dashed line) or hypoglossal nucleus (below dashed line). Hematoxylin and eosin (H&E) stain. B) Raccoon 9 shows mild to moderate spongiform change in the DMNV. H&E stain. C) Raccoon 2 shows very mild PrPSc immunoreactivity in the DMNV and no immunoreactivity in neurons of the hypoglossal nucleus. PrP antibodies F89/160.1.5 and F99/97.6.1. D) Raccoon 9 shows moderate PrPSc immunoreactivity in the neuropil of the DMNV and marked intraneuronal immunoreactivity in the hypoglossal nucleus. PrP antibodies F89/160.1.5 and F99/97.6.1. E–H) Caudate nucleus. E) Raccoon 2 shows moderate diffuse spongiform change. H&E stain. F) Raccoon 9 shows marked diffuse spongiform change. H&E stain. G) Raccoon 2 shows diffuse neuropil PrPSc immunoreactivity and prominent extracellular PrPSc accumulation on neurons (arrowheads). PrP antibodies F89/160.1.5 and F99/97.6.1. H) Raccoon 9 shows marked intracellular PrPSc immunoreactivity in neurons (arrowheads) and glial cells (arrows). PrP antibodies F89/160.1.5 and F99/97.6.1.

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Page created: February 02, 2022
Page updated: March 19, 2022
Page reviewed: March 19, 2022
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