Volume 28, Number 4—April 2022
Research Letter
Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
Table
Results of repeated library preparations and sequencing of 25 random SARS-CoV-2 samples with Omicron variant and mutation previously assigned as P681R, including an alternative chemistry and sequencing platform*
Location |
NYULH Genome Technology Center |
NYSDOH |
|||||
---|---|---|---|---|---|---|---|
Protocol | xGen SARS-CoV-2 Amp Panel 96rxn† | AmpliSeq SARS-CoV-2 Insight‡ | |||||
Reverse transcriptase | SuperScript IV | Maxima H Minus | SuperScript VILO cDNA synthesis kit§ | ||||
PCR cycles, no. P681R calls | |||||||
18, 0 | 18, 0 | 27, 0 | |||||
24, 1 |
24, 1 | NA |
*NA, not applicable; NYSDOH, New York State Department of Health, Albany, NY, USA, which performed ThermoFisher sequencing chemistry on 12 samples; NYULH, NYU Langone Health, New York, NY, USA, which performed Illumina sequencing chemistry on 13 different samples; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. †Integrated DNA Technologies (https://eu.idtdna.com). ‡ThermoFisher Scientific (https://www.thermofisher.com). §Invitrogen (https://www.thermofisher.com).