Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Volume 28, Number 4—April 2022

Research Letter

Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA

Adriana HeguyComments to Author , Dacia Dimartino, Christian Marier, Paul Zappile, Emily Guzman, Ralf Duerr, Guiqing Wang, Jonathan Plitnick, Alexis Russell, Daryl M. Lamson, and Kirsten St. George
Author affiliations: NYU Langone Health, New York, New York, USA (A. Heguy, D. Dimartino, C. Marier, P. Zappile, E. Guzman); NYU Grossman School of Medicine, New York (A. Heguy, R. Duerr, G. Wang); New York State Department of Health, Albany, New York, USA (J. Plitnick, A. Russell, D.M. Lamson, K. St. George); State University of New York at Albany, Albany (K. St. George)

Main Article

Table

Results of repeated library preparations and sequencing of 25 random SARS-CoV-2 samples with Omicron variant and mutation previously assigned as P681R, including an alternative chemistry and sequencing platform*

Location
 NYULH Genome Technology Center
 NYSDOH
Protocol xGen SARS-CoV-2 Amp Panel 96rxn† AmpliSeq SARS-CoV-2 Insight‡
Reverse transcriptase SuperScript IV Maxima H Minus SuperScript VILO cDNA synthesis kit§
PCR cycles, no. P681R calls
18, 0 18, 0 27, 0
24, 1
 24, 1 NA

*NA, not applicable; NYSDOH, New York State Department of Health, Albany, NY, USA, which performed ThermoFisher sequencing chemistry on 12 samples; NYULH, NYU Langone Health, New York, NY, USA, which performed Illumina sequencing chemistry on 13 different samples; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. †Integrated DNA Technologies (https://eu.idtdna.com). ‡ThermoFisher Scientific (https://www.thermofisher.com). §Invitrogen (https://www.thermofisher.com).

Main Article

Page created: January 26, 2022
Page updated: March 19, 2022
Page reviewed: March 19, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external