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Volume 28, Number 5—May 2022
Research

Estimating Relative Abundance of 2 SARS-CoV-2 Variants through Wastewater Surveillance at 2 Large Metropolitan Sites, United States

Alexander T. Yu1Comments to Author , Bridgette Hughes1, Marlene K. Wolfe, Tomas Leon, Dorothea Duong, Angela Rabe, Lauren C. Kennedy, Sindhu Ravuri, Bradley J. White, Krista R. Wigginton, Alexandria B. Boehm, and Duc J. Vugia
Author affiliations: California Department of Public Health, Richmond, California, USA (A.T. Yu, T. Leon, A. Rabe, S. Ravuri, D.J. Vugia); California Department of Public Health, Sacramento, California, USA (A.T. Yu, T. Leon, A. Rabe, S. Ravuri, D.J. Vugia); Verily Life Sciences, South San Francisco, California, USA (B. Hughes, D. Duong, B.J. White); Stanford University, Stanford, California, USA (M.K. Wolfe, L.C. Kennedy, A.B. Boehm); Emory University, Atlanta, Georgia, USA (M.K. Wolfe); University of Michigan, Ann Arbor, Michigan, USA (K.R. Wigginton)

Main Article

Figure 1

Measurements of severe acute respiratory syndrome coronavirus 2 variants of concern in wastewater solids, San Jose, California, USA. Concentrations of N gene and mutations found in Delta (Del156-157/R158G; panel A), and Alpha (HV69-70; panel B) variants in wastewater solids and their ratio (panel C). Error bars in panels A and B represent SDs derived from the 10 replicates run for each sample; open white circles are nondetects (below the limit of detection) and shown as 0. Errors include technical and replication errors. If error bars are not visible, then errors are smaller than the symbol. Panel C shows smoothed lines for visual reference for mutation ratios. For Del156-157/R158G/N ratio, the smoothed line is a 3-point running average, and for the HV69-70/N ratio, the smoothed line is a 7-point running average; each approximates a weekly average. The timescale for the HV69-70 data (B) is truncated for visualization; additional data on dates before February 15, 2021, are described in the article and shown (C), with the exception of data from July 14, 2020, which was nondetect. N, nucleoprotein.

Figure 1. Measurements of severe acute respiratory syndrome coronavirus 2 variants of concern in wastewater solids, San Jose, California, USA. Concentrations of N gene and mutations found in Delta (Del156-157/R158G; panel A), and Alpha (HV69-70; panel B) variants in wastewater solids and their ratio (panel C). Error bars in panels A and B represent SDs derived from the 10 replicates run for each sample; open white circles are nondetects (below the limit of detection) and shown as 0. Errors include technical and replication errors. If error bars are not visible, then errors are smaller than the symbol. Panel C shows smoothed lines for visual reference for mutation ratios. For Del156-157/R158G/N ratio, the smoothed line is a 3-point running average, and for the HV69-70/N ratio, the smoothed line is a 7-point running average; each approximates a weekly average. The timescale for the HV69-70 data (B) is truncated for visualization; additional data on dates before February 15, 2021, are described in the article and shown (C), with the exception of data from July 14, 2020, which was nondetect. N, nucleoprotein.

Main Article

1These first authors contributed equally to this article.

Page created: March 03, 2022
Page updated: April 19, 2022
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