Isolation and Characterization of Novel Reassortant Influenza A(H10N7) Virus in a Harbor Seal, British Columbia, Canada
Yohannes Berhane, Tomy Joseph, Oliver Lung, Carissa Embury-Hyatt, Wanhong Xu, Paul Cottrell, and Stephen Raverty
Author affiliations: University of Manitoba Department of Animal Science, Winnipeg, Manitoba, Canada (Y. Berhane); Canadian Food Inspection Agency, Winnipeg (Y. Berhane, O. Lung, C. Embury-Hyatt, W. Xu); Western College of Veterinary Medicine, Saskatoon, Saskatchewan, Canada (Y. Berhane, S. Raverty); Ministry of Agriculture, Food and Fisheries, Abbotsford, British Columbia, Canada (T. Joseph, S. Raverty); University of Manitoba Department of Biological Sciences, Winnipeg (O. Lung); Fisheries and Aquaculture Management, Vancouver, British Columbia, Canada (P. Cottrell); University of British Columbia Institute for Oceans and Fisheries, Vancouver (S. Raverty)
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Figure 2
Figure 2. Maximum-likelihood phylogenetic tree of influenza A virus subtype H10 hemagglutinin gene from an adult male harbor seal, British Columbia, Canada (red text), and reference sequences. Phylogenetic analyses were based on the full-length nucleotide sequence of the hemagglutinin gene of strains representing the H10 subtype (n = 1,512). The evolutionary relationship was inferred using RAxML (https://github.com/stamatak/standard-RAxML) based on the general time-reversible model with 1,000 bootstrap replicates. For purposes of clarity, some clades are collapsed, and colors are assigned to indicate the origin of the gene: blue for North America, green for Asia, and yellow for Europe. The tree was drawn to scale; branch lengths are measured in number of substitutions per site.
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