Volume 28, Number 8—August 2022
Dispatch
Child Melioidosis Deaths Caused by Burkholderia pseudomallei–Contaminated Borehole Water, Vietnam, 2019
Quyen T.L. Tran, Phuc H. Phan, Linh N.H. Bui, Ha T.V. Bui, Ngoc T.B. Hoang, Dien M. Tran, and Trung T. Trinh
Table 2
Culture results and genotype data from environmental samples in a study of 3 child melioidosis deaths caused by Burkholderia pseudomallei–contaminated borehole water, Vietnam, 2019*
| Sample type, date | No. samples | No. sampling points† | Qualitative culture |
Median quantitative count, CFU (range) | No. isolates selected for MLST‡ | ST | |
|---|---|---|---|---|---|---|---|
| No. (%) positive samples | No. (%) positive sampling points† | ||||||
| Sampling 1, 2019 Nov 17 | |||||||
| Front garden soil | 7 | 7 | 0 | 0 | NP | NA | |
| Water from borehole A | 3 | 1 | 3 (100) | 1 (100) | 2§ | 3 | 541 |
| Water from borehole B | 3 | 1 | 0 | 0 | NP | NA | NA |
| Water from borehole C | 3 | 1 | 0 | 0 | NP | NA | NA |
| Boiled drinking water |
1 |
1 |
0 |
0 |
NP |
NA |
NA |
| Sampling 2, 2019 Nov 23 | |||||||
| Back garden soil near borehole A | 46 | 10 | 27 (58.7) | 8 (80) | 406 (12–746)§ | 6 | 541 |
| Rice field soil | 40 | 8 | 5 (12.5) | 2 (25) | ND | 5 | 541 |
| Water from borehole A | 26 | 1 | 26 (100) | 1 (100) | ND | 4 | 541 |
| Water from borehole B | 3 | 1 | 0 | 0 | NP | NA | NA |
| Water from borehole C | 3 | 1 | 0 | 0 | NP | NA | NA |
| Water from neighbors’ borehole | 33 | 11 | 0 | 0 | NP | NA | NA |
| Water from ponds | 30 | 10 | 0 | 0 | NP | NA | NA |
*CFU, colony forming unit; MLST, multilocus sequence typing; NA, not applicable; ND, not detected; NP, not performed; ST, sequence type. †Sampling points refer to garden, borehole, field, and pond sites. ‡We selected 20 B. pseudomallei isolates for sampling; 2 patient isolates are not shown here. §B. pseudomallei colonies were countable only in 2 borehole water samples and 5 garden soil samples (Appendix). In water samples CFU/mL; in soil samples CFU/g.
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