Volume 28, Number 9—September 2022
Research Letter
Antibodies against SARS-CoV-2 Suggestive of Single Events of Spillover to Cattle, Germany
Table
Results of samples that tested positive by a multispecies SARS-CoV-2 RBD-based ELISA, Germany, 2021*
Cattle farm/animal number (federal state) | RBD-ELISA, corrected OD | Indirect IFA, titer | sVNT, % inhibition |
---|---|---|---|
8/1 (Bavaria) | 0.35 | <1:8 | 6.1 |
11/1 (Bavaria) | 0.70 | 1:32 | 36.4 |
31/1 (Bavaria) | 1.00 | 1:512 | 57.8 |
34/1 (Lower Saxony) | 0.50 | 1:32 | 11.7 |
42/1 (Lower Saxony) | 0.65 | 1:16 | 5.5 |
45/1 (Lower Saxony) | 0.67 | 1:8 | 10.6 |
45/2 (Lower Saxony) | 0.33 | 1:16 | 9.0 |
47/1 (Lower Saxony) | 0.48 | 1:8 | 37.1 |
47/2 (Lower Saxony) | 0.67 | 1:8 | 0.6 |
72/1 (Thuringia) | 0.52 | 1:16 | 4.7 |
74/1 (Thuringia) | 0.76 | 1:32 | 54.2 |
*Boldface indicates positive results. IFA, immunofluorescence assay; OD, optical density; RBD, receptor-binding domain; sVNT, surrogate virus neutralization test (cPass SARS-CoV-2 Surrogate Virus Neutralization Test Kit; GenScript, https://www.genscript.com; cutoff >30% positive and <30% negative).