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Volume 28, Number 9—September 2022
Research Letter

Antibodies against SARS-CoV-2 Suggestive of Single Events of Spillover to Cattle, Germany

Kerstin WernikeComments to Author , Jens Böttcher, Silke Amelung, Kerstin Albrecht, Tanja Gärtner, Karsten Donat, and Martin BeerComments to Author 
Author affiliations: Friedrich-Loeffler-Institut, Greifswald–Insel Riems, Germany (K. Wernike, M. Beer); Bavarian Animal Health Service, Poing, Germany (J. Böttcher); LUFA Nord-West, Oldenburg, Germany (S. Amelung); State Institute for Consumer Protection of Saxony-Anhalt, Stendal, Germany (K. Albrecht); Thuringian Animal Diseases Fund, Animal Health Service, Jena, Germany (T. Gärtner, K. Donat)

Main Article


Results of samples that tested positive by a multispecies SARS-CoV-2 RBD-based ELISA, Germany, 2021*

Cattle farm/animal number (federal state) RBD-ELISA, corrected OD Indirect IFA, titer sVNT, % inhibition
8/1 (Bavaria) 0.35 <1:8 6.1
11/1 (Bavaria) 0.70 1:32 36.4
31/1 (Bavaria) 1.00 1:512 57.8
34/1 (Lower Saxony) 0.50 1:32 11.7
42/1 (Lower Saxony) 0.65 1:16 5.5
45/1 (Lower Saxony) 0.67 1:8 10.6
45/2 (Lower Saxony) 0.33 1:16 9.0
47/1 (Lower Saxony) 0.48 1:8 37.1
47/2 (Lower Saxony) 0.67 1:8 0.6
72/1 (Thuringia) 0.52 1:16 4.7
74/1 (Thuringia) 0.76 1:32 54.2

*Boldface indicates positive results. IFA, immunofluorescence assay; OD, optical density; RBD, receptor-binding domain; sVNT, surrogate virus neutralization test (cPass SARS-CoV-2 Surrogate Virus Neutralization Test Kit; GenScript,; cutoff >30% positive and <30% negative).

Main Article

Page created: July 14, 2022
Page updated: August 19, 2022
Page reviewed: August 19, 2022
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