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Volume 28, Number 9—September 2022
Research Letter

Pathogenesis and Transmissibility of North American Highly Pathogenic Avian Influenza A(H5N1) Virus in Ferrets

Joanna A. Pulit-Penaloza, Jessica A. Belser, Nicole Brock, Poulami Basu Thakur, Terrence M. Tumpey, and Taronna R. MainesComments to Author 
Author affiliation: Author affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article


Results of ferrets inoculated with influenza A(H5N1) virus isolate A/American Wigeon/SC/22–000345–001/2021*

Parameter Value
Weight loss, %†
Temperature increase, °C‡
Nasal wash titer (peak titer days)§
4.6 (1–5)
Virus titer at day 3 postinoculation¶
Nasal wash 2.4 (3/3)
Nasal turbinates 3.5 (3/3)
Soft palate 2.9 (3/3)
Trachea ND
Lung 2.2 (1/3)

*Six ferrets were inoculated with 6 log10 EID50 of A/American Wigeon/SC/22-000345-001/2021 (GISAID identical virus sequence accession no. EPI_ISL_9869760,; in a 1-mL volume. Three additional ferrets were inoculated and then euthanized and tested for virus titer at day 3 postinoculation. EID50, 50% egg infectious dose; ND, not detected †Percentage mean maximum weight loss within 9 days postinoculation. ‡Mean maximum rise in body temperature from baseline (37.6°C–39.4°C). §Mean maximum virus titer in nasal washes, expressed as log10 EID50/mL. ¶Viral titers are expressed as log10 EID50/mL, except for lung titer, which is expressed as log10 EID50/g of tissue. Number of ferrets with detectable virus is specified in parentheses. Limit of virus detection was 1.5 log10 EID50/mL or g.

Main Article

Page created: July 03, 2022
Page updated: August 19, 2022
Page reviewed: August 19, 2022
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