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Volume 29, Number 1—January 2023
Research

Molecular Tools for Early Detection of Invasive Malaria Vector Anopheles stephensi Mosquitoes

Om P. SinghComments to Author , Taranjeet Kaur, Gunjan Sharma, Madhavinadha P. Kona, Shobhna Mishra, Neera Kapoor, and Prashant K. Mallick
Author affiliations: National Institute of Malaria Research, New Delhi, India (O.P. Singh, T. Kaur, G. Sharma, M.P. Kona, S. Mishra, P.K. Mallick); Indira Gandhi National Open University, New Delhi (N. Kapoor)

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Table 1

Sequences of primers and probes used in study of molecular tools for early detection of invasive malaria vector Anopheles stephensi mosquitoes

Name Sequence, 5′ → 3′ Annealing specificity Reference
Stq-F TCTTTCCTCGCATCCAGTTG An. stephensi This study
Stq-R CGGGAGAAGCGGTGATAAAT An. stephensi This study
Stq-P /56-FAM/CGTGCTAAC/ZEN/CTCACTCACCCACAC/3IABKFQ/ An. stephensi This study
Uq-F GAGATTCCCTCTGTCCCTATCT Universal This study
Uq-R AGCTCAACAGGGTCTTCTTTC Universal This study
Uq-P /5HEX/TAGCGAAAC/ZEN/CACAGCCAAGGGAA/3IABKFQ/ Universal This study
U5.8S-F ATCACTCGGCTCATGGATCG Universal (15)
St-F CGTATCTTTCCTCGCATCCA An. stephensi This study
UD2-R GCACTATCAAGCAACACGACT Universal This study
StD2-R GTCTGCCACCACAGTCCT An. stephensi This study

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