Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 29, Number 1—January 2023
Research

Molecular Tools for Early Detection of Invasive Malaria Vector Anopheles stephensi Mosquitoes

Om P. SinghComments to Author , Taranjeet Kaur, Gunjan Sharma, Madhavinadha P. Kona, Shobhna Mishra, Neera Kapoor, and Prashant K. Mallick
Author affiliations: National Institute of Malaria Research, New Delhi, India (O.P. Singh, T. Kaur, G. Sharma, M.P. Kona, S. Mishra, P.K. Mallick); Indira Gandhi National Open University, New Delhi (N. Kapoor)

Main Article

Figure 3

Gel photographs visualizing the result of PCRs specific to invasive malaria vector Anopheles stephensi mosquitoes. A) PCR protocol 1 on individual specimens and pools of An. stephensi and An. culicifacies. B) PCR protocol 2 on larger pools of An. stephensi and An. culicifacies. C) PCR protocol 2 on a pool of 100 mosquitoes containing a single An. stephensi mixed with other wild-caught anophelines (An. culicifacies, An. fluviatilis, An. minimus, and An. subpictus). The numerator of numeric expression shown on the top of each lane indicates number of An. stephensi in a pool, and denominator indicates size of mosquito-pool. L, 100-bp ladder; NC, negative control.

Figure 3. Gel photographs visualizing the result of PCRs specific to invasive malaria vector Anopheles stephensi mosquitoes. A) PCR protocol 1 on individual specimens and pools of An. stephensi and An. culicifacies. B) PCR protocol 2 on larger pools of An. stephensi and An. culicifacies. C) PCR protocol 2 on a pool of 100 mosquitoes containing a single An. stephensi mixed with other wild-caught anophelines (An. culicifacies, An. fluviatilis, An. minimus, and An. subpictus). The numerator of numeric expression shown on the top of each lane indicates number of An. stephensi in a pool, and denominator indicates size of mosquito-pool. L, 100-bp ladder; NC, negative control.

Main Article

Page created: November 10, 2022
Page updated: December 21, 2022
Page reviewed: December 21, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external