Volume 29, Number 1—January 2023
Dispatch
Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
Table 2
Comparison between PCR-positive acute serum samples and paired convalescent serum samples in study using serum specimens for real-time PCR-based diagnosis of human granulocytic anaplasmosis, Canada*
Sample no. | Acute serum samples |
Convalescent serum samples |
Time, d† | Conversion‡ | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
IFA titer | Ct-I | Ct-R | PCR status | IFA | Ct-I | Ct-R | PCR status | ||||
1 | <1:64 | 23.7 | 24.7 | Positive | 1:128 | 40.0 | NA | Negative | 24 | Yes | |
2 | <1:64 | 24.3 | NS | Positive | 1:512 | 35.5 | NS | Positive | 33 | Yes | |
3 | <1:64 | 25.1 | NS | Positive | <1:64 | 27.6 | NS | Positive | 42 | No | |
4 | <1:64 | 25.8 | NS | Positive | 1:1024 | 39.9 | 37.1 | Positive | 13 | Yes | |
5 | <1:64 | 26.8 | NS | Positive | 1:512 | 40.0 | NA | Negative | 56 | Yes | |
6 | 1:64 | 28.0 | 25.3 | Positive | 1:256 | 40.0 | NA | Negative | 49 | Yes | |
7 | 1:2048 | 28.2 | 30.4 | Positive | 1:2048 | 40.0 | NA | Negative | 12 | No | |
8 | <1:64 | 28.8 | 28.1 | Positive | 1:128 | 40.0 | NA | Negative | 21 | Yes | |
9 | <1:64 | 29.7 | NS | Positive | <1:64 | 40.0 | NA | Negative | 39 | No | |
10 | 1:1024 | 31.0 | 29.9 | Positive | 1:1024 | 40.0 | NA | Negative | 28 | No | |
11 | <1:64 | 31.2 | 31.4 | Positive | <1:64 | 40.0 | NA | Negative | 0 | No | |
12 | <1:64 | 31.7 | 31.7 | Positive | 1:256 | 40.0 | NA | Negative | 51 | Yes | |
13 | 1:1024 | 32.4 | 32.8 | Positive | 1:256 | 39.8 | 40.0 | Negative | 8 | No | |
14 | <1:64 | 32.4 | 33.4 | Positive | 1:256 | 40.0 | NA | Negative | 38 | Yes | |
15 | 1:512 | 32.8 | 31.7 | Positive | 1:512 | 40.0 | NA | Negative | 27 | No | |
16 | <1:64 | 34.7 | 36.3 | Positive | <1:64 | 40.0 | NA | Negative | 38 | No | |
17 | 1:1024 | 35.6 | 36.3 | Positive | 1:512 | 40.0 | NA | Negative | 57 | No | |
18 | <1:64 | 36.1 | 37.8 | Positive | 1:64 | 40.0 | NA | Negative | 10 | No | |
19 | <1:64 | 37.5 | 36.8 | Positive | 1:256 | 40.0 | NA | Negative | 46 | Yes |
*Only Ct values <40 were deemed positive. Ct, cycle threshold; Ct-I, initial Ct values (in duplicate); Ct-R, confirmatory Ct values for repeat extraction; IFA, indirect immunofluorescence assay; NA, not applicable; NS, no sample remaining for repeat extraction. †Time difference between acute and convalescent serum sampling. ‡Seroconversion was defined as >4-fold increase in IFA titer between acute and convalescent samples.