Table 1

Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy

Giuseppina La Rosa

, Pamela Mancini, Carolina Veneri, Giusy Bonanno Ferraro, Luca Lucentini, Marcello Iaconelli, and Elisabetta Suffredini

Author affiliation: Istituto Superiore di Sanità, Rome, Italy

Primers and probes used to detect monkeypox virus DNA in airport wastewater, Rome, Italy*

PCR ID	Target	Primer name	Primer ID	Sequence, 5′ → 3′	Position†	Annealing temp. (°C)	Amplicon size	Ref.
1002	G2R	MPVX G F	2368	GGAAAATGTAAAGACAACGAATACAG	194459–84	60	90 bp	(12)
		MPVX G R	2369	GCTATCACATAATCTGGAAGCGTA	194525–48
		MPVX G P	2370	FAM-AAGCCGTAATCTATG TTGTCTATCGTGTCC-BHQ1	194485–514
1005	G2R	MPVX G F mod	2377	GGAAAGTGTAAAGACAACGAATACAG	194459–84	60	90 bp	(12)
		MPVX G R mod	2378	GCTATCACATAATCTGAAAGCGTA	194525–48			This study
		MPVX G P	2370	FAM-AAGCCGTAATCTATG TTGTCTATCGTGTCC-BHQ1	194485–514
1003	F3L	F3L-F290	2371	CTCATTGATTTTTCGCGGGATA	46313–34	60	107 bp	(11)
		F3L-R396	2372	GACGATACTCCTCCTCGTTGGT	46398–419
		F3Lp333S-MGB	2373	FAM-CATCAGAATCTGTAGGCCGT-MGBNFQ	46398–419
1008	F3L	F3L-F290	2371	CTCATTGATTTTTCGCGGGATA	46313–34	60	107 bp	(11)
		F3L-R396 mod	2384	AACGATACTCCTCCTCGTTGGT	46398–419			This study
		F3Lp333S-MGB	2373	FAM-CATCAGAATCTGTAGGCCGT-MGBNFQ	46398–419
1004	N3R	N3R-F319	2374	AACAACCGTCCTACAATTAAACAACA	190641–66	60	139 bp	(11)
		N3R-R457	2375	CGCTATCGAACCATTTTTGTAGTCT	190755–79
		N3Rp352S-MGB	2376	FAM-TATAACGGCGAAGAATATACT-MGBNFQ	190674–94
1016	N3R	N3R-F319	2374	AACAACCGTCCTACAATTAAACAACA	190641–66	60	139 bp	(11)
		N3R-R457	2375	CGCTATCGAACCATTTTTGTAGTCT	190755–79			This study
		N3Rp352S-MGB mod	2381	FAM-TATAACGGCGACGAATATACT-MGBNFQ	190674–94
1006	G2R	G2R-1st cycle F	2379	ATAGCACCACATGCACCATC	194435–54	63	156 bp	This study
		G2R-1st cycle R	2380	AAAGGTATCCGAACCACACG	194590–71
1005	G2R	MPVX G F mod	2377	GGAAAGTGTAAAGACAACGAATACAG	194459–84	61	90 bp	This study
		MPVX G R mod	2378	GCTATCACATAATCTGAAAGCGTA	194525–48
1009	F3L	F3L-1st cycle F	2385	CAGGGTTAACACCTTTCCAA	46242–61	61	212 bp	This study
		F3L-1st cycle R	2386	TGATCTTCAACGTAGTGCTATGG	46453–31
1008	F3L	F3L-F290	2371	CTCATTGATTTTTCGCGGGATA	46313–34	62	107 bp	(11)
		F3L-R396 mod	2384	AACGATACTCCTCCTCGTTGGT	46398–419			This study
1007	N3R	N3R-1st cycle F	2382	TCTATCTCGTTCATGGTCGGTAAT	190503–26	64	455 bp	This study
		N3R-1st cycle R	2383	CGCACTGTCTTATTCGCCATT	190957–37
1004	N3R	N3R-F319	2374	AACAACCGTCCTACAATTAAACAACA	190641–66	64	139 bp	(11)
		N3R-R457	2375	CGCTATCGAACCATTTTTGTAGTCT	190755–79

*Bold underlined text in bases represents modifications to the original primers and probes. ID, identification; F, forward; mod, modified; MPXV, monkeypox virus; R, reverse; ref., reference. †Position based on monkeypox virus reference isolate MPXV_USA_2022_MA001, complete genome, GenBank accession no. ON563414.

Main Article

Page created: November 15, 2022

Page updated: December 22, 2022

Page reviewed: December 22, 2022

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Volume 29, Number 1—January 2023

Dispatch

Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy

Table 1