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Volume 29, Number 11—November 2023
Research Letter

Ceftazidime/Avibactam Resistance in Carbapenemase-Producing Klebsiella pneumoniae

Qiaozhen Cui1, Chen Wang1, Qichen Wang, Juanxiu Qin, Min Li, Baixing DingComments to Author , and Zhen ShenComments to Author 
Author affiliations: Shanxi Provincial People's Hospital, Taiyuan, China (Q. Cui); Renji Hospital at Shanghai Jiao Tong University School of Medicine, Shanghai, China (C. Wang, Q. Wang, J. Qin, M. Li, Z. Shen); Huashan Hospital at Fudan University, Shanghai (B. Ding); Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai (B. Ding)

Main Article

Figure 1

Linear alignment of plasmid pVir-KP0714 and virulence potential determination of Klebsiella. pneumoniae isolate KP0714 in study of ceftazidime/avibactam resistance in carbapenemase-producing K. pneumoniae. A) Virulence potential determination of KP0714 and pVir-KP0714–curing mutant (PC-KP0714) in a mouse infection model. Sequence type 11 carbapenem-resistant hypervirulent K. pneumoniae strain CR-HvKP4 was used as a hypervirulence control. Bacterial suspensions in the logarithmic growth phase were diluted in sterile phosphate-buffered saline to 107 CFU/mL. Six female BALB/c mice were used as a sample population for each isolate. BALB/c mice were infected intraperitoneally with 0.1 mL of the diluted bacterial suspension. Clinical signs and mortality rates were noted for 7 days. *p<0.05 when compared with PC-KP0714. B) Human neutrophil assays of KP0714. Error bars indicate SDs. p values were computed by 1-way analysis of variance with Bonferroni correction.

Figure 1. Linear alignment of plasmid pVir-KP0714 and virulence potential determination of Klebsiella. pneumoniae isolate KP0714 in study of ceftazidime/avibactam resistance in carbapenemase-producing K. pneumoniae. A) Virulence potential determination of KP0714 and pVir-KP0714–curing mutant (PC-KP0714) in a mouse infection model. Sequence type 11 carbapenem-resistant hypervirulent K. pneumoniae strain CR-HvKP4 was used as a hypervirulence control. Bacterial suspensions in the logarithmic growth phase were diluted in sterile phosphate-buffered saline to 107 CFU/mL. Six female BALB/c mice were used as a sample population for each isolate. BALB/c mice were infected intraperitoneally with 0.1 mL of the diluted bacterial suspension. Clinical signs and mortality rates were noted for 7 days. *p<0.05 when compared with PC-KP0714. B) Human neutrophil assays of KP0714. Error bars indicate SDs. p values were computed by 1-way analysis of variance with Bonferroni correction.

Main Article

1These first authors contributed equally to this article.

Page created: September 28, 2023
Page updated: October 23, 2023
Page reviewed: October 23, 2023
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