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Volume 29, Number 2—February 2023
Research

Penicillin and Cefotaxime Resistance of Quinolone-Resistant Neisseria meningitidis Clonal Complex 4821, Shanghai, China, 1965–2020

Mingliang Chen1, Youxing Shao1, Jiayuan Luo, Lingyue Yuan, Minggui WangComments to Author , Min ChenComments to Author , and Qinglan GuoComments to Author 
Author affiliations: Shanghai Institutes of Preventive Medicine, Shanghai, China (Mingliang Chen); Shanghai Municipal Center for Disease Control and Prevention, Shanghai (Mingliang Chen, J. Luo, L. Yuan, Min Chen); Huashan Hospital, Fudan University, Shanghai (Y. Shao, M. Wang, Q. Guo); Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China, Shanghai (Y.Shao, M.G. Wang, Q. Guo)

Main Article

Figure 3

Phylogenetic analysis of penA alleles of Neisseria isolates and genomes, Shanghai, China, 1965–2020, and reference isolates. Phylogenetic analysis of the nucleotide sequences of 580 penA alleles (nucleotides 1321–1722) from N. meningitidis (n = 21,582), N. gonorrhoeae (n = 7,605), N. lactamica (n = 683), N. subflava (n = 431), N. cinerea (n = 65) , N. polysaccharea (n = 52), N. mucosa (n = 33), and other commensal Neisseria (n = 73) isolates and genomes collected in this study and from the Neisseria PubMLST database was constructed by using IQ-TREE version 2.2.0 (23), with both SH-aLRT test and UFboot set as 1,000. The values of SH-aLRT and ultrafast bootstrap (Ufboot) are shown on the node of each clade as SH-aLRT/Ufboot. Clusters were determined by using SH-aLRT values of 80% from the SH-aLRT tests with 1,000 replicates and ultrafast bootstrap (UFBoot) values of 85% from bootstrap tests with 1,000 replicates (IQ-TREE). Alleles penA378, penA405, penA552, penA553, penA843, penA868, and penA917 were within in the N. lactamica cluster; penA662, penA777, and penA865 were within the N. subflava cluster; penA379 was within the N. gonorrhoeae cluster; and the other 8 penA alleles were located outside the 5 clusters. Scale bar indicates substitutions per site. PenNS, penicillin-nonsusceptible meningococci.

Figure 3. Phylogenetic analysis of penA alleles of Neisseria isolates and genomes, Shanghai, China, 1965–2020, and reference isolates. Phylogenetic analysis of the nucleotide sequences of 580 penA alleles (nucleotides 1321–1722) from N. meningitidis (n = 21,582), N. gonorrhoeae (n = 7,605), N. lactamica (n = 683), N. subflava (n = 431), N. cinerea (n = 65) , N. polysaccharea (n = 52), N. mucosa (n = 33), and other commensal Neisseria (n = 73) isolates and genomes collected in this study and from the Neisseria PubMLST database was constructed by using IQ-TREE version 2.2.0 (23), with both SH-aLRT test and UFboot set as 1,000. The values of SH-aLRT and ultrafast bootstrap (Ufboot) are shown on the node of each clade as SH-aLRT/Ufboot. Clusters were determined by using SH-aLRT values of 80% from the SH-aLRT tests with 1,000 replicates and ultrafast bootstrap (UFBoot) values of 85% from bootstrap tests with 1,000 replicates (IQ-TREE). Alleles penA378, penA405, penA552, penA553, penA843, penA868, and penA917 were within in the N. lactamica cluster; penA662, penA777, and penA865 were within the N. subflava cluster; penA379 was within the N. gonorrhoeae cluster; and the other 8 penA alleles were located outside the 5 clusters. Scale bar indicates substitutions per site. PenNS, penicillin-nonsusceptible meningococci.

Main Article

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1These authors contributed equally to this article.

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