Álvaro A. Faccini-Martínez, Carlos Ramiro Silva-Ramos, Lucas S. Blanton, Esteban Arroyave, Heidy-C. Martínez-Diaz, Paola Betancourt-Ruiz, Marylin Hidalgo, and David H. Walker
Figure. Serologic evidence of Orientia infection among rural population, Cauca Department, Colombia. Western blot analysis was performed using Vero cell extracts and O. tsutsugamushi Karp strain whole-cell antigens. Examples of some reactive samples that were applied to probe-migrating Orientia-specific antigens (56 kD and 47 kD) are shown: A) samples H479 and H499; B) samples H478 and H503. Red arrows indicate 56-kD protein, and blue stars indicate 47-kD protein. OTK, O. tsutsugamushi Karp strain; PL, protein ladder; VC, Vero cell; (‒) C, negative control; (+) C, positive control.