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Volume 29, Number 4—April 2023
Research Letter

Rickettsia conorii Subspecies israelensis in Captive Baboons

Giovanni Sgroi, Roberta Iatta, Grazia Carelli, Annamaria Uva, Maria Alfonsa Cavalera, Piero Laricchiuta, and Domenico OtrantoComments to Author 
Author affiliations: Experimental Zooprophylactic Institute of Southern Italy, Portici, Italy (G. Sgroi); University of Bari Aldo Moro, Bari, Italy (G. Sgroi, R. Iatta, G. Carelli, A. Uva, M.A. Cavalera, D. Otranto); Zoosafari Park Fasano, Brindisi, Italy (P. Laricchiuta); Bu-Ali Sina University, Hamedan, Iran (D. Otranto)

Main Article

Table

PCR protocols used in study of vector-borne pathogens among baboons, Italy, 2020

Pathogen Target gene Primer Sequence, 5′ →3′ Fragment length, bp Reference
Babesia/Theileria spp. 18S rRNA RLB-F GAGGTAGTGACAAGAAATAACAATA 460–520 (2)


RLB-R
TCTTCGATCCCCTAACTTTC


Ehrlichia/Anaplasma spp. 16S rRNA EHR-16SD GGTACCYACAGAAGAAGTCC 345 (2)


HER-16SR
TAGCACTCATCGTTTACAGC


Rickettsia spp. gltA CS-78F GCAAGTATCGGTGAGGATGTAAT 401 (2)


CS-323R
GCTTCCTTAAAATTCAATAAATCAGGAT


Spotted fever group Rickettsiae ompA Rr190.70F ATGGCGAATATTTCTCCAAAA 632 (2)
Rr190.701R GTTCCGTTAATGGCAGCATCT
ompB 120–2788 AAACAATAATCAAGGTACTGT 600 (3)


120–3599
TACTTCCGGTTACAGCAAAGT


Leishmania infantum kDNA minicircle Leish-1 AACTTTTCTGGTCCTCCG GGTAG 120 (4)
Leish-2 ACCCCCAGTTTCCCGCC

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References
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