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Volume 29, Number 5—May 2023
Research

Leishmania donovani Transmission Cycle Associated with Human Infection, Phlebotomus alexandri Sand Flies, and Hare Blood Meals, Israel1

Liora StudentskyComments to Author , Laor Orshan, Fouad Akad, Irina Ben Avi, Debora Diaz, Shirly Elbaz, Orly Sagi, Gal Zagron, Lea Valinsky, Maya Davidovich-Cohen, and Gad Baneth
Author affiliations: Ministry of Health Central Laboratories for Public Health, Jerusalem, Israel (L. Studentsky, L. Orshan, I. Ben Avi, D. Diaz, S. Elbaz, M. Davidovich-Cohen); The Hebrew University of Jerusalem, Rehovot, Israel (L. Studentsky, G. Baneth); Ministry of Health National Laboratory for Public Health, Tel Aviv, Israel (F. Akad); Soroka University Medical Center, Beer-Sheba, Israel (O. Sagi); Israeli Ministry of Environmental Protection, Jerusalem (G. Zagron); Maccabi Healthcare Services, Tel Aviv (L. Valinsky)

Main Article

Figure 3

Phylogenetic analysis of entire Leishmania internal transcribed spacer region in study of Leishmania donovani transmission cycle associated with human infection, Phlebotomus alexandri sand flies, and hare blood meals, Israel. Leishmania-specific internal transcribed spacer region (988 bp) was amplified by PCR from P. alexandri sand flies, pooled female Phlebotomus spp. flies, and patient samples and then sequenced. Tree was constructed by using by using the maximum-likelihood method and Tamura 3-parameter model of all relevant Leishmania spp. and Trypanosoma cruzi as an outgroup. Sand fly and clinical samples from this study (black triangles), L. infantum isolates from Israel (black circles), Leishmania international reference strains (empty circles), and available GenBank Leishmania sequences are shown. GenBank accession numbers, isolate source, and country of origin are shown for each sequence. Only bootstrap values >70% are shown next to branches. Not to scale.

Figure 3. Phylogenetic analysis of entire Leishmania internal transcribed spacer region in study of Leishmania donovani transmission cycle associated with human infection, Phlebotomus alexandri sand flies, and hare blood meals, Israel. Leishmania-specific internal transcribed spacer region (988 bp) was amplified by PCR from P. alexandri sand flies, pooled female Phlebotomus spp. flies, and patient samples and then sequenced. Tree was constructed by using by using the maximum-likelihood method and Tamura 3-parameter model of all relevant Leishmania spp. and Trypanosoma cruzi as an outgroup. Sand fly and clinical samples from this study (black triangles), L. infantum isolates from Israel (black circles), Leishmania international reference strains (empty circles), and available GenBank Leishmania sequences are shown. GenBank accession numbers, isolate source, and country of origin are shown for each sequence. Only bootstrap values >70% are shown next to branches. Not to scale.

Main Article

1Data from this study were presented at the Israeli Society for Parasitology, Protozoology and Tropical Diseases Annual Meeting; March 21, 2022; Kfar Hamaccabiah, Ramat Gan, Israel.

Page created: March 06, 2023
Page updated: April 18, 2023
Page reviewed: April 18, 2023
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