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Volume 29, Number 9—September 2023
Dispatch

Anaplasma bovis–Like Infections in Humans, United States, 2015–2017

Sandor E. KarpathyComments to Author , Luke Kingry, Bobbi S. Pritt, Jonathan C. Berry, Neil B. Chilton, Shaun J. Dergousoff1, Roberto Cortinas, Sarah W. Sheldon, Stephanie Oatman2, Melissa Anacker, Jeannine Petersen, and Christopher D. Paddock
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (S.E. Karpathy, C.D. Paddock); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (L. Kingry, S.W. Sheldon, S. Oatman, J. Petersen); Mayo Clinic, Rochester, Minnesota, USA (B.S. Pritt, J.C. Berry); University of Saskatchewan, Saskatoon, Saskatchewan, Canada (N.B. Chilton, S.J. Dergousoff); University of Nebraska, Lincoln, Nebraska, USA (R. Cortinas); Minnesota Department of Health, St. Paul, Minnesota, USA (M. Anacker)

Main Article

Figure

Phylogenetic relationship of novel human Anaplasma bovis–like pathogen associated with human cases in the United States, 2015–2017, to other A. bovis–like and related Anaplasma species based on 2,039 bp of concatenated rrs, gltA, groEL nucleotide sequences. Phylogenetic relationships were inferred using the RAxML method using the general time reversible plus gamma model (13). One thousand bootstrap replicates were used to estimate the likelihood of the tree; bootstrap values are displayed next to the nodes. Only bootstrap values of >50 are shown. GenBank accession numbers for the samples in this study: OQ772254;, gltA; OQ772255, groEL; and OQ724830, rrs; those for the D. andersoni sample were assigned the following numbers: OQ772256, gltA; OQ772257, groEL; and OQ724821, rrs. Reference sequences from GenBank: Anaplasma bovis (cow, China): MH255937, 16S; MH594290, gltA; MH255906.1, groEL; A. bovis (goat, China): MH255939, 16S; MH255915.1, gltA; MH255907, groEL; A. bovis (raccoon, Japan): GU937020, 16S; JN588561, gltA; JN588562, groEL; Anaplasma platys strain Okinawa: AY077619, 16S; AY077620, gltA; AY077621, groEL; A. phagocytophilum strain HZ NC_007797; A. centrale strain Israel NC_013532; A. marginale strain Florida NC_012026. Ehrlichia chaffeensis strain West Paces (NZ_CP007480) was used as the outgroup. Scale bar represents mean number of nucleotide substitutions per site.

Figure. Phylogenetic relationship of novel human Anaplasma bovis–like pathogen associated with human cases in the United States, 2015–2017, to other A. bovis–like and related Anaplasma species based on 2,039 bp of concatenated rrs, gltA, groEL nucleotide sequences. Phylogenetic relationships were inferred using the RAxML method using the general time reversible plus gamma model (13). One thousand bootstrap replicates were used to estimate the likelihood of the tree; bootstrap values are displayed next to the nodes. Only bootstrap values of >50 are shown. GenBank accession numbers for the samples in this study: OQ772254;, gltA; OQ772255, groEL; and OQ724830, rrs; those for the D. andersoni sample were assigned the following numbers: OQ772256, gltA; OQ772257, groEL; and OQ724821, rrs. Reference sequences from GenBank: Anaplasma bovis (cow, China): MH255937, 16S; MH594290, gltA; MH255906.1, groEL; A. bovis (goat, China): MH255939, 16S; MH255915.1, gltA; MH255907, groEL; A. bovis (raccoon, Japan): GU937020, 16S; JN588561, gltA; JN588562, groEL; Anaplasma platys strain Okinawa: AY077619, 16S; AY077620, gltA; AY077621, groEL; A. phagocytophilum strain HZ NC_007797; A. centrale strain Israel NC_013532; A. marginale strain Florida NC_012026. Ehrlichia chaffeensis strain West Paces (NZ_CP007480) was used as the outgroup. Scale bar represents mean number of nucleotide substitutions per site.

Main Article

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1Current affiliation: Agriculture and Agri-food Canada, Lethbridge, Alberta, Canada.

2Current affiliation: Mayo Clinic, Jacksonville, Florida, USA.

Page created: July 11, 2023
Page updated: August 20, 2023
Page reviewed: August 20, 2023
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