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Volume 29, Number 9—September 2023
Dispatch

Anaplasma bovis–Like Infections in Humans, United States, 2015–2017

Sandor E. KarpathyComments to Author , Luke Kingry, Bobbi S. Pritt, Jonathan C. Berry, Neil B. Chilton, Shaun J. Dergousoff1, Roberto Cortinas, Sarah W. Sheldon, Stephanie Oatman2, Melissa Anacker, Jeannine Petersen, and Christopher D. Paddock
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (S.E. Karpathy, C.D. Paddock); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (L. Kingry, S.W. Sheldon, S. Oatman, J. Petersen); Mayo Clinic, Rochester, Minnesota, USA (B.S. Pritt, J.C. Berry); University of Saskatchewan, Saskatoon, Saskatchewan, Canada (N.B. Chilton, S.J. Dergousoff); University of Nebraska, Lincoln, Nebraska, USA (R. Cortinas); Minnesota Department of Health, St. Paul, Minnesota, USA (M. Anacker)

Main Article

Table 1

PCR primers used in study of Anaplasma bovis–like infections in humans, United States, 2015–2017

Gene Primer name Sequence, 5′ → 3′ Annealing temperature* Reference
rrs Out2F GAT AGC GGA ATT CCT AGT GTA GAG GTG 56°C (8)
317Pan
 AAA GGA GGT AAT CCA GC
gltA Abov_gltA2F CGG AAA TTA CTT TTA TAG ATG G 49°C This study
Abov_gltA2R
 CAT ACC AYT GAG AAA CCC AAC
groEL HS1-f CGT CAG TGG GCT GGT AAT GAA 54°C (9,10)
HS6-r CCW CCW GGT CWA CAC CTT C 50°C (11)
HS3-f ATA GTY ATG AAG GAG AGT GAT
HSVR TCA ACA GCA GCT CTA GTW G

*Cycling conditions: 95°C for 3 min followed by 35 cycles of 95°C for 30 s, 1 min at the annealing temperature listed above, and an extension at 72°C for 1 min 30 s. This was followed by a final extension at 72°C for 10 min.

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1Current affiliation: Agriculture and Agri-food Canada, Lethbridge, Alberta, Canada.

2Current affiliation: Mayo Clinic, Jacksonville, Florida, USA.

Page created: July 11, 2023
Page updated: August 20, 2023
Page reviewed: August 20, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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