Figure 1. Amyloid formation rates in fecal samples from white-tailed deer with chronic wasting disease in longitudinal study of temporal characterization of prion shedding in secreta, United States. A) Amyloid formation rates measured by using iron oxide bead capture and subsequent real-time quaking-induced conversion. Rates were measured for serial dilutions of fecal samples. B) Amyloid formation rates measured by using iron oxide bead capture, 4 rounds of serial protein misfolding amplification, and subsequent real-time quaking-induced conversion. Rates were measured for 1:100 dilutions of fecal samples from each round of amplification. Black indicates prion-negative feces; red indicates prion-positive feces. Horizontal lines in each grouping indicate median values. Rd, round.