Figure 2. Amyloid formation rates in urine and saliva samples from white-tailed deer with chronic wasting disease in longitudinal study of temporal characterization of prion shedding in secreta, United States. A) Amyloid formation rates measured by using iron oxide bead capture and subsequent real-time quaking-induced conversion. Black indicates prion-negative samples; red indicates prion-positive samples. B) Amyloid formation rates measured by using iron oxide bead capture, 4 rounds of serial protein misfolding amplification, and subsequent real-time quaking-induced conversion in the same samples as those in panel A. Rates were measured for samples after amplification rounds 2–4. Horizontal lines in each grouping indicate median values. Rd, round.