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Volume 30, Number 12—December 2024
Dispatch

Feline Panleukopenia Virus in a Marsican Brown Bear and Crested Porcupine, Italy, 2022–2023

Georgia Diakoudi, Gianvito Lanave, Shadia Berjaoui, Costantina Desario, Giovanni Di Teodoro, Violetta Iris Vasinioti, Francesco Pellegrini, Sabrina V.P. Defourny, Stefania Salucci, Antonio Cocco, Alessio Lorusso, Vito Martella, and Nicola DecaroComments to Author 
Author affiliation: University of Bari Aldo Moro, Bari, Italy (G. Diakoudi, G. Lanave, C. Desario, V.I. Vasinioti, F. Pellegrini, V. Martella, N. Decaro); Istituto Zooprofilattico Sperimentale di Abruzzo e Molise ‘Giuseppe Caporale,’ Teramo, Italy (S. Berjaoui, G. Di Teodoro, S.V.P. Defourny, S. Salucci, A. Cocco, A. Lorusso); University of Veterinary Medicine, Budapest, Hungary (V. Martella)

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Table 2

Oligonucleotides used for the multiplex PCR protocols based on ARTIC-like strategy used in study of feline panleukopenia virus in wild animals, Abruzzo and Molise, Italy, January 2022–May 2023*

Primer Sequence, 5′ → 3′ Amplicon size, bp
CPV1_left AATGATAGGCGGTTTGTGTGTTT 396
CPV1_right CAACTTCCGATTCCCAGTCCAT 396
CPV2_left CCAATTCAAAATGAAGAGCTAACATCTT 410
CPV2_right GTCACCCATTCACTATCTTCTGCA 410
CPV3_left TGGAGTAGATGGTTGGTGACTCT 416
CPV3_right ACCAAGTCCCGCAAAGTACATT 416
CPV4_left AGCACACTTTACACTGAACAAATGA 401
CPV4_right GCTATAGCGTGACAAACTTTAATCCA 401
CPV5_left AGCAAGAACAAAAACAGCATTTGAA 402
CPV5_right TGATCAATTCTAATTGTTTGTCCAGAACA 402
CPV6_left AAAAATTTAATTTGGATTGAAGAAGCTGGT 407
CPV6_right CCTTCTTGTATTTTAGGCTCCGC 407
CPV7_left TGAATCAACCATGGCTAACTATACACA 393
CPV7_right GGAGGTGCCATCGTACCTTAATC 393
CPV8_left TGGTCCGAAATAGAGGCAGACC 393
CPV8_right CCCCCAATCTTTAGCGTCCTTA 393
CPV9_left GCTGCTTATCTTCGCTCTGGTA 412
CPV9_right AAATCCCCACACCCCCAGAA 412
CPV10_left GCACCAATGAGTGATGGAGCA 410
CPV10_right TCACTCATAGTATTAACAATTAGTTGCCA 410
CPV11_left TGCACAAATTGTAACACCTTGGT 388
CPV11_right ATTTGTTGGTGTGCCACTAGTTC 388
CPV12_left ACCAACCATACCAACTCCATGG 411
CPV12_right TAACCAACCTCAGCTGGTCTCA 411
CPV13_left GGAGTTCAACAAGATAAAAGACGTGG 396
CPV13_right ACCTCCAATTGGATCTGTTGGT 396
CPV14_left TCCAGAAGGAGATTGGATTCAAAATATT 411
CPV14_right TTCCAAGTATGAGAGGCTCTTAGTT 411
CPV15_left TTGATACTGACTTAAAACCAAGACTTCA 511
CPV15_right ACAGTTATTGTATACCATATAACAAACCTTCT 511

*ARTIC-like strategy as shown in (5). CPV, canine parvovirus.

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