Volume 30, Number 12—December 2024
Research Letter
Zoonotic Potential of Chronic Wasting Disease after Adaptation in Intermediate Species
, Sylvie L. Benestad, Jean-Yves Douet, Alvina Huor, Séverine Lugan, Naïma Aron, Hervé Cassard, Juan Carlos Espinosa, Alicia Otero, Rosa Bolea, Juan María Torres, and Olivier Andréoletti
Table
Transmission of a moose CWD isolate in a study of zoonotic potential of chronic wasting disease after adaptation in intermediate species*
| Model characteristics |
TgMet |
TgVal |
Tg338 |
BoTg110 |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No./ no.† |
Mean dpi (SD) |
PrPres band type |
No./ no.† |
Mean dpi (SD) |
PrPres band type |
No./ no.† |
Mean dpi (SD) |
PrPres band type |
No./ no.† |
Mean dpi (SD) |
PrPres band type |
|||||
| Prion strains | ||||||||||||||||
| M1CJD (sCJD MM1) | ||||||||||||||||
| 1st passage | 6/6 | 219 (17) | 21 kDa | 6/6 | 327 (19)‡ | 21 kDa | ND | ND | ||||||||
| 2nd passage |
6/6 |
239 (8)‡ |
21 kDa |
6/6 |
286 (16)‡ |
21 kDa |
ND |
ND |
||||||||
| V2CJD (sCJD VV2) | ||||||||||||||||
| 1st passage | 6/6 | 618 (81)‡ | 21 kDa | 6/6 | 168 (12)‡ | 19 kDa | ND | ND | ||||||||
| 2nd passage |
6/6 |
509 (41)‡ |
21 kDa |
6/6 |
169 (12)‡ |
19 kDa |
ND |
ND |
||||||||
| Classical BSE | ||||||||||||||||
| 1st passage | 1/12 | 739‡ | BSE§ | 0/12 | >750‡ | NA | 6/6 | >750¶ | BSE§ | 6/6 | 295 (12)# | BSE§ | ||||
| 2nd passage |
9/12 |
613 (43)‡ |
BSE§ |
0/12 |
>750‡ |
NA |
6/6 |
682 (52)¶ |
BSE§ |
6/6 |
265 (35)# |
BSE§ |
||||
| Sheep-adapted BSE | ||||||||||||||||
| 1st passage | 6/6 | 690 (83)# | BSE§ | ND | 6/6 | >750¶ | BSE§ | 6/6 | 254 (19)¶ | BSE§ | ||||||
| 2nd passage |
5/5 |
564 (39)# |
BSE§ |
ND |
6/6 |
653 (32)¶ |
BSE§ |
6/6 |
234 (12)¶ |
BSE§ |
||||||
| Tg338-adapted BSE | ||||||||||||||||
| 1st passage | 6/6 | 596 (92) | BSE§ | 0/6 | >700 | BSE§ | 5/5 | 224 (37) | BSE§ | 6/6 | 222 (22) | BSE§ | ||||
| 2nd passage |
ND |
ND |
ND |
|||||||||||||
| Moose CWD (16–60-P153) | ||||||||||||||||
| 1st passage | 0/12 | >700 | NA | 0/12 | >700 | NA | 2/12 | 612, 717 | 19 kDa, 21 kDa | 0/12 | >700 | NA | ||||
| 2nd passage |
0/6 | >700 | NA | ND | 5/5 | 167 (4)** | 21 kDa | ND | ||||||||
| ND |
ND |
6/6 |
244 (33)†† |
21 kDa |
ND |
|||||||||||
| Tg338-adapted moose CWD‡‡ | ||||||||||||||||
| 1st passage | 1/8 | 561 | 19+21 kDa | 5/6 | 483 (35) | 21 kDa | 7/7 | 95 (5) | 21 kDa | 5/5 | 431 (32) | 20 kDa | ||||
| 2nd passage | ND | 4/4 | 311 (12) |
21 kDa | ND | ND | ||||||||||
*Results show moose isolate (16–60-P153) from Norway and reference prion strains (human sCJD strains M1CJD and V2CJD, cattle strain c-BSE) in transgenic mouse models expressing human PrPC 129M (TgMet) and 129V (TgVal), ovine VRQ PrPC (Tg338) and bovine PrPC (BoTg110). BSE, bovine spongiform encephalopathy; dpi, days post-inoculation; NA, not available; ND, not done; PrP, prion protein; PrPres, PK-resistant prion protein; VRQ, valine136-arginine154-glutamine171 ovine PrPC variant. †No. affected mice/total no. inoculated. ‡Transmissions reported in (5) §Transmissions reported in (6) ¶Transmissions reported in (7) #Transmissions reported in (8) **Transmission was performed from the brain of the first-passage mouse that showed a 19 kDa banding pattern. ††Transmission was performed from the brain of the first-passage mouse that showed a 21 kDa banding pattern. ‡‡The Tg338-adapted isolate corresponds to the brain of a second-passage Tg338 mouse that was culled at 170 dpi after infection with first-passage 19K brain.