Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 30, Number 5—May 2024
Dispatch

Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

Abd’jeleel Salawudeen, Geoff Soule, Nikesh Tailor, Levi Klassen, Jonathan Audet, Angela Sloan, Yvon Deschambault, and David SafronetzComments to Author 
Author affiliations: University of Manitoba, Winnipeg, Manitoba, Canada (A. Salawudeen, D. Safronetz); Public Health Agency of Canada, Winnipeg (G. Soule, N. Tailor, L. Klassen, J. Audet, A. Sloan, Y. Deschambault, D. Safronetz)

Main Article

Figure 2

Protective efficacy of lyophilized vesicular stomatitis virus–based vaccines in guinea pig model. A) Body temperatures; B) weight changes; C) survival; D) virus titrations in different tissues. Groups of 10 Hartley guinea pigs each were immunized with VSV∆G/LASVGPC vaccine or lyophilized/reconstituted Ly-VSV∆G/LASVGPC or Ly-VSV∆G/EBOVGP. Ly-VSV∆G/EBOVGP was used as the sham-vaccinated inoculum control group. Animals were challenged 28 days after immunization with a lethal dose of guinea pig–adapted Lassa virus Josiah strain. Disease progression was monitored in 6 animals in each group; the remaining 4 animals per group were euthanized on day 13 postinfection for analysis of infectious Lassa virus in tissues. LOD, limit of detection; Ly-VSV∆G/EBOVGP, lyophilized vesicular stomatitis virus expressing Ebola virus glycoprotein; Ly-VSV∆G/LASVGPC, lyophilized vesicular stomatitis virus expressing Lassa virus glycoprotein; TCID50, 50% tissue culture infectious dose; VSV∆G/LASVGPC, vesicular stomatitis virus expressing Lassa virus glycoprotein.

Figure 2. Protective efficacy of lyophilized vesicular stomatitis virus–based vaccines in guinea pig model. A) Body temperatures; B) weight changes; C) survival; D) virus titrations in different tissues. Groups of 10 Hartley guinea pigs each were immunized with VSV∆G/LASVGPC vaccine or lyophilized/reconstituted Ly-VSV∆G/LASVGPC or Ly-VSV∆G/EBOVGP. Ly-VSV∆G/EBOVGP was used as the sham-vaccinated inoculum control group. Animals were challenged 28 days after immunization with a lethal dose of guinea pig–adapted Lassa virus Josiah strain. Disease progression was monitored in 6 animals in each group; the remaining 4 animals per group were euthanized on day 13 postinfection for analysis of infectious Lassa virus in tissues. LOD, limit of detection; Ly-VSV∆G/EBOVGP, lyophilized vesicular stomatitis virus expressing Ebola virus glycoprotein; Ly-VSV∆G/LASVGPC, lyophilized vesicular stomatitis virus expressing Lassa virus glycoprotein; TCID50, 50% tissue culture infectious dose; VSV∆G/LASVGPC, vesicular stomatitis virus expressing Lassa virus glycoprotein.

Main Article

Page created: March 05, 2024
Page updated: April 24, 2024
Page reviewed: April 24, 2024
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external