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Volume 30, Number 8—August 2024
Research

Geographic Distribution of Rabies Virus and Genomic Sequence Alignment of Wild and Vaccine Strains, Kenya

Evalyne N. Wambugu, Gathii Kimita, Sarah N. Kituyi, Michael A. Washington, Clement Masakhwe, Lucy M. Mutunga, Gurdeep Jaswant, S.M. Thumbi, Brian C. Schaefer, and John N. WaitumbiComments to Author 
Author affiliations: Walter Reed Army Institute of Research–Africa, Kenya Medical Research Institute, Kisumu, Kenya (E.N. Wambugu, G. Kimita, C. Masakhwe, J.N. Waitumbi); University of Embu, Embu, Kenya (E.N. Wambugu, S.N. Kituyi); Dwight D. Eisenhower Army Medical Center, Augusta, Georgia, USA (M.A. Washington); National Institutes of Health, Bethesda, Maryland, USA (M.A. Washington); University of Nairobi, Nairobi, Kenya (L.M. Mutunga, G. Jaswant, S.M. Thumbi); Washington State University, Pullman, Washington, USA (S.M. Thumbi); Uniformed Services University, Bethesda (B.C. Schaefer)

Main Article

Figure 2

Phylogenetic tree of virus genomes from a study of geographic distribution of rabies virus and genomic sequence alignment of wild and vaccine strains, Kenya. The tree was constructed from whole-genome sequences by using MAD DOG (44). All genomes were of Cosmopolitan clade, subclades Africa-1a or Africa-1b, and were further delineated into a total of 14 distinct lineages, 8 from western Kenya and 5 in eastern Kenya. One lineage from eastern Kenya was unclassified. Scale bar indicates nucleotide substitutions per site.

Figure 2. Phylogenetic tree of virus genomes from a study of geographic distribution of rabies virus and genomic sequence alignment of wild and vaccine strains, Kenya. The tree was constructed from whole-genome sequences by using MAD DOG (44). All genomes were of Cosmopolitan clade, subclades Africa-1a or Africa-1b, and were further delineated into a total of 14 distinct lineages, 8 from western Kenya and 5 in eastern Kenya. One lineage from eastern Kenya was unclassified. Scale bar indicates nucleotide substitutions per site.

Main Article

References
  1. Steele  JH, Fernandez  PJ. History of rabies and global aspects. In: Baer GM, editor. The natural history of rabies. Boca Raton (FL): CRC Press; 2017. p. 1–24.
  2. Dunlop  RH, Williams  DJ. Veterinary medicine: an illustrated history. St. Louis (MO): Mosby-Year Book, Inc.; 1996.
  3. Talbi  C, Holmes  EC, de Benedictis  P, Faye  O, Nakouné  E, Gamatié  D, et al. Evolutionary history and dynamics of dog rabies virus in western and central Africa. J Gen Virol. 2009;90:78391. DOIPubMedGoogle Scholar
  4. Schnell  MJ, McGettigan  JP, Wirblich  C, Papaneri  A. The cell biology of rabies virus: using stealth to reach the brain. Nat Rev Microbiol. 2010;8:5161. DOIPubMedGoogle Scholar
  5. Yousaf  MZ, Qasim  M, Zia  S, Khan  M, Ashfaq  UA, Khan  S. Rabies molecular virology, diagnosis, prevention and treatment. Virol J. 2012;9:50. DOIPubMedGoogle Scholar
  6. Fisher  CR, Streicker  DG, Schnell  MJ. The spread and evolution of rabies virus: conquering new frontiers. Nat Rev Microbiol. 2018;16:24155. DOIPubMedGoogle Scholar
  7. Walker  PJ, Freitas-Astúa  J, Bejerman  N, Blasdell  KR, Breyta  R, Dietzgen  RG, et al.; Ictv Report Consortium. ICTV virus taxonomy profile: Rhabdoviridae 2022. J Gen Virol. 2022;103:001689. DOIPubMedGoogle Scholar
  8. Müller  T, Freuling  CM. Rabies in terrestrial animals. In: Fooks AR, Jackson AC, editors. Rabies: scientific basis of the disease and its management, 4th edition. London: Elsevier Academic Press; 2020. p. 195–230.
  9. Shipley  R, Wright  E, Lean  FZX, Selden  D, Horton  DL, Fooks  AR, et al. Assessing rabies vaccine protection against a novel lyssavirus, Kotalahti bat lyssavirus. Viruses. 2021;13:947. DOIPubMedGoogle Scholar
  10. World Health Organization. WHO expert consultation on rabies. WHO technical report series no. 931. Geneva: The Organization; 2005.
  11. Scott  TP, Nel  LH. Lyssaviruses and the fatal encephalitic disease rabies. Front Immunol. 2021;12:786953. DOIPubMedGoogle Scholar
  12. Singh  R, Singh  KP, Cherian  S, Saminathan  M, Kapoor  S, Manjunatha Reddy  GB, et al. Rabies - epidemiology, pathogenesis, public health concerns and advances in diagnosis and control: a comprehensive review. Vet Q. 2017;37:21251. DOIPubMedGoogle Scholar
  13. Meske  M, Fanelli  A, Rocha  F, Awada  L, Soto  PC, Mapitse  N, et al. Evolution of rabies in South America and inter-species dynamics (2009–2018). Trop Med Infect Dis. 2021;6:98. DOIPubMedGoogle Scholar
  14. Slate  D, Algeo  TP, Nelson  KM, Chipman  RB, Donovan  D, Blanton  JD, et al. Oral rabies vaccination in north america: opportunities, complexities, and challenges. PLoS Negl Trop Dis. 2009;3:e549. DOIPubMedGoogle Scholar
  15. World Health Organization. WHO expert consultation on rabies. Second report. World Health Organ Tech Rep Ser. 2013;982:1139.PubMedGoogle Scholar
  16. Zhou  M, Zhou  Z, Kia  GSN, Gnanadurai  CW, Leyson  CM, Umoh  JU, et al. Complete genome sequence of a street rabies virus isolated from a dog in Nigeria. Genome Announc. 2013;1:e0021412. DOIPubMedGoogle Scholar
  17. Kuzmin  IV, Shi  M, Orciari  LA, Yager  PA, Velasco-Villa  A, Kuzmina  NA, et al. Molecular inferences suggest multiple host shifts of rabies viruses from bats to mesocarnivores in Arizona during 2001-2009. PLoS Pathog. 2012;8:e1002786. DOIPubMedGoogle Scholar
  18. Caraballo  DA, Lema  C, Novaro  L, Gury-Dohmen  F, Russo  S, Beltrán  FJ, et al. A novel terrestrial rabies virus lineage occurring in South America: origin, diversification, and evidence of contact between wild and domestic cycles. Viruses. 2021;13:2484. DOIPubMedGoogle Scholar
  19. Troupin  C, Dacheux  L, Tanguy  M, Sabeta  C, Blanc  H, Bouchier  C, et al. Large-scale phylogenomic analysis reveals the complex evolutionary history of rabies virus in multiple carnivore hosts. PLoS Pathog. 2016;12:e1006041. DOIPubMedGoogle Scholar
  20. Bourhy  H, Reynes  J-M, Dunham  EJ, Dacheux  L, Larrous  F, Huong  VTQ, et al. The origin and phylogeography of dog rabies virus. J Gen Virol. 2008;89:267381. DOIPubMedGoogle Scholar
  21. Brunker  K, Hampson  K, Horton  DL, Biek  R. Integrating the landscape epidemiology and genetics of RNA viruses: rabies in domestic dogs as a model. Parasitology. 2012;139:1899913. DOIPubMedGoogle Scholar
  22. Sadeuh-Mba  SA, Momo  JB, Besong  L, Loul  S, Njouom  R. Molecular characterization and phylogenetic relatedness of dog-derived Rabies Viruses circulating in Cameroon between 2010 and 2016. PLoS Negl Trop Dis. 2017;11:e0006041. DOIPubMedGoogle Scholar
  23. Bitek  AO, Osoro  E, Munyua  PM, Nanyingi  M, Muthiani  Y, Kiambi  S, et al. A hundred years of rabies in Kenya and the strategy for eliminating dog-mediated rabies by 2030. AAS Open Res. 2019;1:23. DOIPubMedGoogle Scholar
  24. McDermott  JJ, Kitala  PM. The epidemiology of rabies in Machakos District, Kenya. Nairobi: University of Nairobi College of Agriculture and Veterinary Studies; 2003.
  25. Chuchu  VM, Kitala  PM, Bichanga  P, Ksee  D, Muturi  M, Mwatondo  A, et al. Rabies elimination in rural Kenya: need for improved availability of human vaccines, awareness and knowledge on rabies and its management among healthcare workers. Front Public Health. 2022;10:769898. DOIPubMedGoogle Scholar
  26. Republic of Kenya, Ministry of Health and Ministry of Agriculture, Livestock and Fisheries. Strategic plan for the elimination of human rabies in Kenya 2014–2030. Nairobi: The Republic; 2014.
  27. Hampson  K, Coudeville  L, Lembo  T, Sambo  M, Kieffer  A, Attlan  M, et al.; Global Alliance for Rabies Control Partners for Rabies Prevention. Estimating the global burden of endemic canine rabies. PLoS Negl Trop Dis. 2015;9:e0003709. DOIPubMedGoogle Scholar
  28. World Health Organization. WHO Expert Consultation on Rabies, WHO technical report series, no. 1012. Geneva: The Organization; 2018.
  29. World Health Organization. Rabies vaccines: WHO position paper, April 2018 - Recommendations. Vaccine. 2018;36:55003. DOIPubMedGoogle Scholar
  30. Luo  Y, Zhang  Y, Liu  X, Yang  Y, Yang  X, Zhang  D, et al. Complete genome sequence of a highly virulent rabies virus isolated from a rabid pig in south China. J Virol. 2012;86:124545. DOIPubMedGoogle Scholar
  31. Zhang  G, Fu  ZF. Complete genome sequence of a street rabies virus from Mexico. J Virol. 2012;86:108923. DOIPubMedGoogle Scholar
  32. Wunner  WH, Conzelmann  K-K. Rabies virus. In: Fooks AR, Jackson AC, editors. Rabies: scientific basis of the disease and its management, 4th edition. London: Elsevier Academic Press; 2020. p. 43–81.
  33. Wang  W, Ma  J, Nie  J, Li  J, Cao  S, Wang  L, et al. Antigenic variations of recent street rabies virus. Emerg Microbes Infect. 2019;8:158492. DOIPubMedGoogle Scholar
  34. Morimoto  K, Hooper  DC, Carbaugh  H, Fu  ZF, Koprowski  H, Dietzschold  B. Rabies virus quasispecies: implications for pathogenesis. Proc Natl Acad Sci U S A. 1998;95:31526. DOIPubMedGoogle Scholar
  35. de Almeida  GL, Cargnelutti  JF, Ries  AS, Ferreira  JC, Rosa  JCA, Batista  HBCR, et al. Sequence analysis of nucleoprotein gene reveals the co-circulation of lineages and sublineages of rabies virus in herbivorous in Rio Grande do Sul state, Brazil. Braz J Microbiol. 2020;51:83746. DOIPubMedGoogle Scholar
  36. Zandi  F, Goshadrou  F, Meyfour  A, Vaziri  B. Rabies infection: an overview of lyssavirus–host protein interactions. Iran Biomed J. 2021;25:22642. DOIPubMedGoogle Scholar
  37. Brightman  C. Rabies: an acute viral infection. Trends Urol Men’s Heal. 2012;3:313. DOIGoogle Scholar
  38. Ajorloo  M, Mirzaei  H, Sadeghi  Y, Tarban  N, Soltani  S, Mohammadi  FS, et al. Evaluation and phylogenetic analysis of regular rabies virus vaccine strains. Arch Iran Med. 2018;21:10110.PubMedGoogle Scholar
  39. Kim  H-H, Yang  D-K, Nah  J-J, Song  J-Y, Cho  I-S. Comparison of the protective efficacy between single and combination of recombinant adenoviruses expressing complete and truncated glycoprotein, and nucleoprotein of the pathogenic street rabies virus in mice. Virol J. 2017;14:122. DOIPubMedGoogle Scholar
  40. Zhang  G, Wang  H, Mahmood  F, Fu  ZF. Rabies virus glycoprotein is an important determinant for the induction of innate immune responses and the pathogenic mechanisms. Vet Microbiol. 2013;162:60113. DOIPubMedGoogle Scholar
  41. Tenzin  T, Lhamo  K, Rai  PB, Tshering  D, Jamtsho  P, Namgyal  J, et al. Evaluation of a rapid immunochromatographic test kit to the gold standard fluorescent antibody test for diagnosis of rabies in animals in Bhutan. BMC Vet Res. 2020;16:183. DOIPubMedGoogle Scholar
  42. Bohlander  SK, Espinosa  R III, Le Beau  MM, Rowley  JD, Díaz  MO. A method for the rapid sequence-independent amplification of microdissected chromosomal material. Genomics. 1992;13:13224. DOIPubMedGoogle Scholar
  43. Wang  D, Coscoy  L, Zylberberg  M, Avila  PC, Boushey  HA, Ganem  D, et al. Microarray-based detection and genotyping of viral pathogens. Proc Natl Acad Sci U S A. 2002;99:1568792. DOIPubMedGoogle Scholar
  44. Campbell  K, Gifford  RJ, Singer  J, Hill  V, O’Toole  A, Rambaut  A, et al. Making genomic surveillance deliver: A lineage classification and nomenclature system to inform rabies elimination. PLoS Pathog. 2022;18:e1010023. DOIPubMedGoogle Scholar
  45. Minh  BQ, Schmidt  HA, Chernomor  O, Schrempf  D, Woodhams  MD, von Haeseler  A, et al. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic ea. Mol Biol Evol. 2020;37:15304. DOIPubMedGoogle Scholar
  46. Kat  PW, Alexander  KA, Smith  JS, Munson  L. Rabies and African wild dogs in Kenya. Proc Biol Sci. 1995;262:22933. DOIPubMedGoogle Scholar
  47. Brunker  K, Jaswant  G, Thumbi  SM, Lushasi  K, Lugelo  A, Czupryna  AM, et al. Rapid in-country sequencing of whole virus genomes to inform rabies elimination programmes. Wellcome Open Res. 2020;5:3. DOIPubMedGoogle Scholar
  48. Gigante  CM, Yale  G, Condori  RE, Costa  NC, Long  NV, Minh  PQ, et al. Portable rabies virus sequencing in canine rabies endemic countries using the Oxford Nanopore MinION. Viruses. 2020;12:1255. DOIPubMedGoogle Scholar
  49. Wheeler  DC, Waller  LA. Mountains, valleys, and rivers: The transmission of raccoon rabies over a heterogeneous landscape. J Agric Biol Environ Stat. 2008;13:388406. DOIPubMedGoogle Scholar
  50. Metlin  A, Paulin  L, Suomalainen  S, Neuvonen  E, Rybakov  S, Mikhalishin  V, et al. Characterization of Russian rabies virus vaccine strain RV-97. Virus Res. 2008;132:2427. DOIPubMedGoogle Scholar

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