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Volume 30, Number 9—September 2024
Research Letter

Confirmed Case of Autochthonous Human Babesiosis, Hungary

Dávid SiposComments to Author , Ágnes Kappéter, Barbara Réger, Gabriella Kiss, Nóra Takács, Róbert Farkas, István Kucsera, and Zoltán Péterfi
Author affiliations: University of Pécs, Pécs, Hungary (D. Sipos, Á. Kappéter, B. Réger, G. Kiss, Z. Péterfi); University of Veterinary Medicine, Budapest, Hungary (N. Takács, R. Farkas); National Public Health Center, Budapest (I. Kucsera)

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Figure 2

Phylogenetic analysis of Babesia spp. in confirmed case of autochthonous human babesiosis, Hungary. Asterisk indicates B. microti isolated from the patient in this case study. Phylogenetic tree was constructed by using Ggtree (5) according to multiple sequence alignments created by using MAFFT software (6). Best substitution model (3-parameter model, TPM2u) was selected by using functions of the phangorn version 2.11.1 R package (7) according to the Bayesian information criterion. Neighbor-joining tree was optimized by using the maximum-likelihood method. Bootstrap values were produced by 100 iterations and are indicated at branches. All data processing and plotting were performed in R version 4.4.1 (The R Project for Statistical Computing, https://www.r-project.org). GenBank accession numbers are indicated after the species name. Scale bar indicates nucleotide substitutions per site.

Figure 2. Phylogenetic analysis of Babesia spp. in confirmed case of autochthonous human babesiosis, Hungary. Asterisk indicates B. microti isolated from the patient in this case study. Phylogenetic tree was constructed by using Ggtree (5) according to multiple sequence alignments created by using MAFFT software (6). Best substitution model (3-parameter model, TPM2u) was selected by using functions of the phangorn version 2.11.1 R package (7) according to the Bayesian information criterion. Neighbor-joining tree was optimized by using the maximum-likelihood method. Bootstrap values were produced by 100 iterations and are indicated at branches. All data processing and plotting were performed in R version 4.4.1 (The R Project for Statistical Computing, https://www.r-project.org). GenBank accession numbers are indicated after the species name. Scale bar indicates nucleotide substitutions per site.

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