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Volume 30, Number 9—September 2024
Research Letter

Thelazia callipaeda Eyeworms in American Black Bear, Pennsylvania, USA, 2023

Caroline SobotykComments to Author , Jaclyn Dietrich, Guilherme G. Verocai, Lauren Maxwell, and Kevin Niedringhaus
Author affiliations: University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA (C. Sobotyk, J. Dietrich, L. Maxwell, K. Niedringhaus); Texas A&M University College of Veterinary Medicine and Biomedical Sciences, College Station, Texas, USA (Guilherme G. Verocai)

Main Article

Figure 2

Phylogenetic relationship of Thelazia callipaeda isolate from an American black bear in Coolbaugh Township, Monroe County, Pennsylvania, USA, 2023 (GenBank accession no. PP739308), and other species of Thelazia available in GenBank (accession numbers shown). Analysis was performed by using the maximum-likelihood method (1,000 bootstrap replicates) in MEGA X version 11 (https://www.megasoftware.net). The best-fit nucleotide substitution model for the dataset was Tamura-Nei with a discrete gamma distribution, which was used to model evolutionary rate differences among sites (5 categories [+G, parameter = 0.2578]). That analysis involved 30 nucleotide sequences. There were 647 positions in the final dataset. Distances, defined as the number of nucleotide substitutions/site, were calculated by using that model. Branches corresponding to partitions reproduced in <50% of bootstrap replicates are collapsed.

Figure 2. Phylogenetic relationship of Thelazia callipaeda isolate from an American black bear in Coolbaugh Township, Monroe County, Pennsylvania, USA, 2023 (GenBank accession no. PP739308), and other species of Thelazia available in GenBank (accession numbers shown). Analysis was performed by using the maximum-likelihood method (1,000 bootstrap replicates) in MEGA X version 11 (https://www.megasoftware.net). The best-fit nucleotide substitution model for the dataset was Tamura-Nei with a discrete gamma distribution, which was used to model evolutionary rate differences among sites (5 categories [+G, parameter = 0.2578]). That analysis involved 30 nucleotide sequences. There were 647 positions in the final dataset. Distances, defined as the number of nucleotide substitutions/site, were calculated by using that model. Branches corresponding to partitions reproduced in <50% of bootstrap replicates are collapsed.

Main Article

Page created: July 30, 2024
Page updated: August 21, 2024
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