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Volume 31, Number 2—February 2025
Dispatch

Detection of Chronic Wasting Disease Prions in Raw, Processed, and Cooked Elk Meat, Texas, USA

Rebeca Benavente, Fraser Brydon, Francisca Bravo-Risi, Paulina Soto, J. Hunter Reed, Mitch Lockwood, Glenn Telling, Marcelo A. Barria, and Rodrigo MoralesComments to Author 
Author affiliation: The University of Texas Health Science Center at Houston, Houston, Texas, USA (R. Benavente, F. Bravo-Risi, P. Soto, R. Morales); University of Edinburgh, Scotland, UK (F. Brydon, M.A. Baria); Universidad Bernardo O’Higgins, Santiago, Chile (F. Bravo-Risi, P. Soto, R. Morales); Texas Park and Wildlife Department, Kerrville, Texas, USA (J.H. Reed, M. Lockwood); Colorado State University, Fort Collins, Colorado, USA (G. Telling)

Main Article

Figure 1

Flowchart of experimental strategy to detect chronic wasting disease prions in raw, processed, and cooked elk meat. We sampled ham, boneless steak, sausage, and hamburger meat from an elk that tested positive for chronic wasting disease. We homogenized raw meat samples and samples of meat cooking by grilling or boiling. We then tested homogenates of raw and cooked meat and the remnant water used in the boiling procedure by PMCA using elk and human substrates. PMCA, protein misfolding cyclic amplification.

Figure 1. Flowchart of experimental strategy to detect chronic wasting disease prions in raw, processed, and cooked elk meat. We sampled ham, boneless steak, sausage, and hamburger meat from an elk that tested positive for chronic wasting disease. We homogenized raw meat samples and samples of meat cooking by grilling or boiling. We then tested homogenates of raw and cooked meat and the remnant water used in the boiling procedure by PMCA using elk and human substrates. PMCA, protein misfolding cyclic amplification.

Main Article

Page created: December 30, 2024
Page updated: January 17, 2025
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