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Volume 31, Number 2—February 2025
Dispatch

Detection of Chronic Wasting Disease Prions in Raw, Processed, and Cooked Elk Meat, Texas, USA

Rebeca Benavente, Fraser Brydon, Francisca Bravo-Risi, Paulina Soto, J. Hunter Reed, Mitch Lockwood, Glenn Telling, Marcelo A. Barria, and Rodrigo MoralesComments to Author 
Author affiliation: The University of Texas Health Science Center at Houston, Houston, Texas, USA (R. Benavente, F. Bravo-Risi, P. Soto, R. Morales); University of Edinburgh, Scotland, UK (F. Brydon, M.A. Baria); Universidad Bernardo O’Higgins, Santiago, Chile (F. Bravo-Risi, P. Soto, R. Morales); Texas Park and Wildlife Department, Kerrville, Texas, USA (J.H. Reed, M. Lockwood); Colorado State University, Fort Collins, Colorado, USA (G. Telling)

Main Article

Figure 2

Western blot of PMCA used for detection of chronic wasting disease prions in raw, processed, and cooked elk meat. A) Representative PMCA data of the different samples tested. The panels depict the results obtained in 3 PMCA rounds of 1 of the replicates conducted in this analysis. All samples were treated with proteinase K, except for PrPC that was used as a control for antibody activity and electrophoretic mobility. Numbers at the right depict molecular weight markers. B) Summary of the PMCA detection data in both replicates for raw, grilled, and boiled meats and for the water used in the boiling process. PMCA, protein misfolding cyclic amplification; PrPC, cellular prion protein; R, round.

Figure 2. Western blot of PMCA used for detection of chronic wasting disease prions in raw, processed, and cooked elk meat. A) Representative PMCA data of the different samples tested. The panels depict the results obtained in 3 PMCA rounds of 1 of the replicates conducted in this analysis. All samples were treated with proteinase K, except for PrPC that was used as a control for antibody activity and electrophoretic mobility. Numbers at the right depict molecular weight markers. B) Summary of the PMCA detection data in both replicates for raw, grilled, and boiled meats and for the water used in the boiling process. PMCA, protein misfolding cyclic amplification; PrPC, cellular prion protein; R, round.

Main Article

Page created: December 30, 2024
Page updated: January 31, 2025
Page reviewed: January 31, 2025
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