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Volume 31, Number 3—March 2025
Research

Genomic Characterization of Circulating Dengue Virus, Ethiopia, 2022–2023

Adugna Abera1Comments to Author , Houriiyah Tegally1Comments to Author , Geremew Tasew, Eduan Wilkinson, Abraham Ali, Feyisa Regasa, Molalegne Bitew, Mahlet Belachew, Lucious Chabuka, Gaspary Mwanyika, Derek Tshiabuila, Jennifer Giandhari, Sureshnee Pillay, Jenicca Poogavanan, Monika Moir, Moritz U.G. Kraemer, Kamran Khan, Carmen Huber, Getachew Tollera, Tobias F. Rinke de Wit, Cheryl Baxter, Richard Lessells, Dawit Wolday, Dereje Beyene2, Tulio de Oliveira2, and CLIMADE Consortium3
Author affiliation: Ethiopian Public Health Institute, Addis Ababa, Ethiopia (A. Abera, G. Tasew, A. Ali, F. Regasa, M. Belachew, G. Tollera, D. Wolday); Addis Ababa University, Addis Ababa (A. Abera, D. Beyene); Centre for Epidemic Response and Innovation, School of Data Science and Computational Thinking, Stellenbosch University, Stellenbosch, South Africa (H. Tegally, E. Wilkinson, L. Chabuka, G. Mwanyika, D. Tshiabuila, J. Poogavanan, M. Moir, C. Baxter, T. de Oliveira); Bio and Emerging Technology Institute, Addis Ababa (M. Bitew); University of KwaZulu-Natal, Durban, South Africa (J. Giandhari, S. Pillay, R. Lessells, T. de Oliveira); University of Oxford, Oxford, UK (M.U.G. Kraemer); University of Toronto, Toronto, Ontario, Canada (K. Khan); BlueDot, Toronto (K. Khan, C. Huber); University of Amsterdam, Amsterdam, the Netherlands (T.F. Rinke de Wit); McMaster University, Hamilton, Ontario, Canada (D. Wolday)

Main Article

Figure 4

Sequencing process and coverage results in study of genomic characterization of circulating DENV, Ethiopia, 2022–2023. A) Patient sample selection, genotyping, and sequencing workflow. B) PCR cycle thresholds compared with sequence coverage for all sequenced specimens from Afar Regions and Dire Dawa. DENV, dengue virus; III-A, genotype III lineage A; III-B, genotype III lineage B; RT-PCR, reverse transcription PCR.

Figure 4. Sequencing process and coverage results in study of genomic characterization of circulating DENV, Ethiopia, 2022–2023. A) Patient sample selection, genotyping, and sequencing workflow. B) PCR cycle thresholds compared with sequence coverage for all sequenced specimens from Afar Regions and Dire Dawa. DENV, dengue virus; III-A, genotype III lineage A; III-B, genotype III lineage B; RT-PCR, reverse transcription PCR.

Main Article

1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

3Members of the CLIMADE consortium are listed in the Appendix.

Page created: January 02, 2025
Page updated: February 28, 2025
Page reviewed: February 28, 2025
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