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Volume 31, Number 4—April 2025
Research Letter

Neutralizing Antibody Response to Influenza A(H5N1) Virus in Dairy Farm Workers, Michigan, USA

Min Z. LevineComments to Author , Feng Liu, Natasha Bagdasarian, Crystal Holiday, Stacie Jefferson, Zhu-Nan Li, Claudia Pappas, Terrence Tumpey, Timothy M. Uyeki, Alexandra M. Mellis, Krista Kniss, Joseph Coyle, Seth Eckel, Jeremy Kuo, Meghan Weinberg, Sarah Lyon-Callo, Lisa Mikesell, Becky Stoddard, and Jennifer Morse
Author affiliation: Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.Z. Levine, F. Liu, C. Holiday, S. Jefferson, Z.-N. Li, C. Pappas, T. Tumpey, T.M. Uyeki, A.M. Mellis, K. Kniss); Michigan Department of Health and Human Services, Lansing, Michigan, USA (N. Bagdasarian, J. Coyle, S. Eckel, J. Kuo, M. Weinberg, S. Lyon-Callo); Mid-Michigan District Health Department, Stanton, Michigan, USA (L. Mikesell, B. Stoddard, J. Morse)

Main Article

Figure

Results of microneutralization and HI assays to assess neutralizing antibody response to HPAI A(H5N1) virus infection in dairy farm workers, Michigan, USA. A) Case MI-A; B) case MI-B. We collected paired serum samples (S1 and S2) and tested against wild-type HPAI H5N1 clade 2.3.4.4b virus. Serum samples were tested at 1:10 predilution, and titer <10 was reported as 5. Dashed lines denote seropositive (titer >40) threshold at 40. GMT, geometric mean titer; HI, hemagglutination inhibition; HPAI, highly pathogenic avian influenza; S1, serum sample 1 (acute phase); S2, serum sample 2 (convalescent phase).

Figure. Results of microneutralization and HI assays to assess neutralizing antibody response to HPAI A(H5N1) virus infection in dairy farm workers, Michigan, USA. A) Case MI-A; B) case MI-B. We collected paired serum samples (S1 and S2) and tested against wild-type HPAI H5N1 clade 2.3.4.4b virus. Serum samples were tested at 1:10 predilution, and titer <10 was reported as 5. Dashed lines denote seropositive (titer >40) threshold at 40. GMT, geometric mean titer; HI, hemagglutination inhibition; HPAI, highly pathogenic avian influenza; S1, serum sample 1 (acute phase); S2, serum sample 2 (convalescent phase).

Main Article

Page created: March 03, 2025
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