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Volume 31, Number 5—May 2025
Dispatch

Molecular Detection of Histoplasma in Bat-Inhabited Tunnels of Camino de Hierro Tourist Route, Spain

Joaquina María García-Martín1, Julio David Soto López1, Diego Lizana-Ciudad, Pedro Fernández-SotoComments to Author , and Antonio Muro
Author affiliation: Universidad de Salamanca, Salamanca, Spain (J.M. García-Martín, J.D. Soto López, D. Lizana-Ciudad, P. Fernández-Soto, A. Muro); Instituto de Investigación Biomédica de Salamanca, Salamanca (J.M. García-Martín, J.D. Soto López, P. Fernández-Soto, A. Muro); Centro de Estudios Ambientales y Dinamización Rural, Salamanca (D. Lizana-Ciudad)

Main Article

Table

Identities of Histoplasma isolates obtained from guano samples collected in the tunnels of the Camino de Hierro tourist route in study of molecular detection of Histoplasma, Spain*

Isolate
code		 Isolate BLAST identity (sublineage)† Isolate accession no. Source species for guano samples Source accession no.
H1 H. suramericanum (LAm A1) PP887829 Rhinolophus ferrumequinum PP919660
H2 H. suramericanum (LAm A1) PP887830 Myotis blythii PP919661
H3 H. suramericanum (LAm A1) PP887831 Myotis sp. PP919662
H4 H. capsulatum variant duboisii/H. capsulatum sensu lato (LAm B2) PP887832 M. blythii PP919663
H5 H. suramericanum (LAm A1) PP887833 Myotis sp. PP919664
H6 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887834 M. blythii PP919665
H7 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887835 M. blythii PP919666
H8 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887836 Myotis sp. PP919667
H9 H. suramericanum (LAm A1) PP887837 M. blythii PP919668
H10 H. suramericanum (LAm A1) PP887838 NA NA
H11 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887839 Miniopterus schreibersii PP919669
H12 H. suramericanum (LAm A1) PP887840 M. blythii PP919670
H13 H. suramericanum (LAm A1) PP887841 M. blythii PP919671
H14 H. suramericanum (LAm A1) PP887842 M. blythii PP919672
H15 H. suramericanum (LAm A1) PP887843 M. blythii PP919673
H16 H. suramericanum (LAm A1) PP887844 M. blythii PP919674
H17 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887845 Myotis sp. PP919675
H18 H. suramericanum (LAm A1) PP887846 M. blythii PP919676
H19 H. suramericanum (LAm A1) PP887847 M. blythii PP919677
H21 H. suramericanum (LAm A1) PP887849 Myotis sp. PP919679
H22 H. suramericanum (LAm A1) PP887850 Myotis sp. PP919680
H23 H. suramericanum (LAm A1) PP887851 M. blythii PP919681
H26 H. suramericanum (LAm A1) PP887854 Myotis sp. PP919684
H27 H. suramericanum (LAm A1) PP887855 M. blythii PP919685
H28 H. suramericanum (LAm A1) PP887856 M. blythii PP919686
H29 H. suramericanum (LAm A1) PP887857 R. ferrumequinum PP919687
H30 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887858 M. blythii PP919688
H31 H. suramericanum (LAm A1) PP887859 NA NA
H32 H. suramericanum (LAm A1) PP887860 M. blythii PP919689
H33 H. suramericanum (LAm A1) PP887861 M. blythii PP919690
H34 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887862 Myotis sp. PP919691
H35 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887863 R. ferrumequinum PP919692
H36 H. suramericanum (LAm A1) PP887864 Myotis sp. PP919693
H37 H. capsulatum sensu stricto PP887865 M. blythii PP919694
H38 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887866 Myotis sp. PP919695
H39 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887867 M. blythii PP919696
H40 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887868 M. schreibersii PP919697
H41 H. suramericanum (LAm A1) PP887869 M. blythii PP919698
H42 H. capsulatum var. duboisii/H. capsulatum s.l. (LAm B2) PP887870 M. blythii PP919699
H43 H. capsulatum s.s. PP887871 M. blythii PP919700
H49 H. suramericanum (LAm A1) PP887877 M. blythii PP919706
H50 H. suramericanum (LAm A1) PP887878 M. blythii PP919707

*GenBank accession numbers are shown; further details are provided in Appendix Table 4. NA, not available. †Species names, according to (2), followed by sublineage when applicable. H. capsulatum s.s. = H. capsulatum Panama or H81 lineage; H. capsulatum var. duboisii = H. capsulatum African lineage or H. duboisii; H. suramericanum = H. capsulatum LAm A. Note that the identity of some isolates could not be determined at species level because the corresponding Hcp100 sequences shared high similarity values with several GenBank isolates representing different Histoplasma species or lineages (separated by a slash).

Main Article

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Main Article

1These first authors contributed equally to this article.

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