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Volume 31, Number 5—May 2025
Dispatch

Administration of L-type Bovine Spongiform Encephalopathy to Macaques to Evaluate Zoonotic Potential

Morikazu Imamura1Comments to Author , Ken’ichi Hagiwara, Minoru Tobiume, Minako Ohno, Hiromi Iguchi, Hanae Takatsuki, Tsuyoshi Mori, Ryuichiro Atarashi, Hiroaki Shibata, and Fumiko Ono1
Author affiliation: University of Miyazaki, Miyazaki, Japan (M. Imamura, M. Ohno, H. Iguchi, H. Takatsuki, T. Mori, R. Atarashi); National Institute of Infectious Diseases, Tokyo, Japan (K. Hagiwara, M. Tobiume); The Corporation for Production and Research of Laboratory Primates, Tsukuba, Japan (H. Shibata); Okayama University of Science, Imabari, Japan (F. Ono)

Main Article

Table

Detection of PrPres in tissue samples obtained from macaques orally challenged with L-BSE prions in study of oral transmission of L-type bovine spongiform encephalopathy in macaques to evaluate zoonotic potential*

Sample location
 PMCA, no. positive/no. total
WB, orally challenged animals
IHC, orally challenged animals
Orally challenged animals
Negative controls
18
 19
9086
 1004
 9040
 18
 19
 18
 19
Nerve tissues
Cerebral cortex, frontal lobe; PTA, PBS 0/2, 0/2 0/2, 0/2 ND 0/2, 0/2 0/2, 0/2 Neg Neg Neg Neg
Cerebral cortex, temporal lobe ND ND ND ND ND Neg Neg Neg Neg
Cerebral cortex, parietal lobe ND ND ND ND ND Neg Neg Neg Neg
Cerebral cortex, occipital lobe ND ND ND ND ND Neg Neg Neg Neg
Hippocampus ND ND ND ND ND Neg Neg Neg Neg
Thalamus ND ND ND ND ND Neg Neg Neg Neg
Cerebellum ND ND ND ND ND Neg Neg Neg Neg
Cervical cord; EtOH, PBS 0/2, 0/2 0/2, 1/2 0/2, 0/2 0/2, 0/2 0/2, 0/2 Neg Neg Neg Neg
Thoracic cord; EtOH, PBS 0/2, 1/2 0/2, 1/2 0/2, 0/2 0/2, 0/2 0/2, 0/2 Neg Neg Neg Neg
Lumbar cord; EtOH, PBS 0/2, 0/2 0/2, 0/2 0/2, 0/2 0/2, 0/2 0/2, 0/2 Neg Neg Neg Neg
Median nerve, PTA 2/2 1/2 ND ND ND Neg Neg Neg Neg
Sciatic nerve, PTA
 0/2
 0/2
ND
 ND
 ND
Neg
 Neg
Neg
 Neg
Secondary lymphoid tissues
Spleen, PTA 1/2 0/2 ND ND 0/2 Neg Neg Neg Neg
Tonsil, PTA 1/2 2/2 0/2 0/2 0/2 ND ND ND ND
Submandibular lymph node, PTA 0/2 0/2 0/2 ND 0/2 Neg Neg Neg Neg
Inguinal lymph nodes, PTA 1/2 2/2 ND ND ND ND ND ND ND
Mesenteric lymph node, PTA
 1/2
 1/2
0/2
 0/2
 0/2
ND
 ND
ND
 ND
Primary lymphoid tissues
Thymus, PTA
 0/2
 2/2
ND
 ND
 ND
ND
 ND
ND
 ND
Others
Submaxillary gland, PTA 2/2 1/2 ND ND ND ND ND ND ND
Ileum, PTA
 2/2
 2/2
0/2
 0/2
 0/2
Neg
 Neg
Neg
 Neg
*Bold indicates positive results. EtOH, ethanol precipitation; IHC, immunohistochemistry; ND, not determined; neg, negative; PBS, suspension in phosphate-buffered saline; PMCA, protein misfolding cyclic amplification; PrPres, PrPSc-like proteinase K–resistant prion proteins; PTA, sodium phosphotungstic acid precipitation; WB, western blot.

Main Article

1These authors were co–principal investigators.

Page created: March 21, 2025
Page updated: April 15, 2025
Page reviewed: April 15, 2025
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