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Volume 31, Number 7—July 2025

Dispatch

Detection of Novel Orthobunyavirus Reassortants in Fatal Neurologic Case in Horse and Culicoides Biting Midges, South Africa

Matshepo Elizabeth Rakaki, Miné van der Walt, June Williams, and Marietjie VenterComments to Author 
Author affiliation: University of Pretoria, Pretoria, South Africa (M.E. Rakaki, J. Williams, M. Venter); University of the Witwatersrand, Johannesburg, South Africa (M. van der Walt, M. Venter)

Main Article

Figure 3

Phylogenetic analysis of Orthobunyavirus L segments in study of reassortants in fatal neurologic case in horse and Culicoides biting midges, South Africa. Tree shows phylogenetic analysis of the positive animal cases and Culicoides pools for the L segment of sequences from South Africa (black dots) relative to orthobunyavirus reference strains available in GenBank within the Simbu serogroup. Tree was constructed in MEGA X (https://www.megasoftware.net) by using the Tamura-Nei model. Bootstrap values from 1,000 replicates are shown at each node. Pacuvirus genus was included as an outgroup to root the tree. Bolded accession numbers in parentheses indicate sequences from this study that were submitted to GenBank. Scale bar indicates nucleotide substitutions per site.

Figure 3. Phylogenetic analysis of Orthobunyavirus L segments in study of reassortants in fatal neurologic case in horse and Culicoides biting midges, South Africa. Tree shows phylogenetic analysis of the positive animal cases and Culicoides pools for the L segment of sequences from South Africa (black dots) relative to orthobunyavirus reference strains available in GenBank within the Simbu serogroup. Tree was constructed in MEGA X (https://www.megasoftware.net) by using the Tamura-Nei model. Bootstrap values from 1,000 replicates are shown at each node. Pacuvirus genus was included as an outgroup to root the tree. Bolded accession numbers in parentheses indicate sequences from this study that were submitted to GenBank. Scale bar indicates nucleotide substitutions per site.

Main Article

Page created: May 14, 2025
Page updated: June 18, 2025
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