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Volume 31, Number 7—July 2025
Dispatch
Detection of Novel Orthobunyavirus Reassortants in Fatal Neurologic Case in Horse and Culicoides Biting Midges, South Africa
Matshepo Elizabeth Rakaki, Miné van der Walt, June Williams, and Marietjie Venter
Table
Orthobunyaviruses identified by PCR and sequencing of S, M, and L segments in samples from mammals and Culicoides biting midges, South Africa*
| Sample no. | Orthobunyavirus RT-PCR S segment | Conventional nested PCR |
||
|---|---|---|---|---|
| S segment | M segment | L segment | ||
| Mammals | ||||
| ZRU053/20 | Shuni virus | IS | IS | IS |
| ZRU012/21 | Shuni virus | Shuni virus | Shamonda virus | Shamonda virus |
| ZRU027/21 | Shuni virus | AF | AF | AF |
| ZRU093/21 | Shamonda virus | Shamonda virus | Shamonda virus | Shamonda virus |
| ZRU019/22 | Shuni virus | Shuni virus | AF | AF |
| ZRU099/22 |
Shuni virus |
Shuni virus |
AF |
Shuni virus |
| Culicoides biting midges | ||||
| MAR538_16 | Schmallenberg virus | Schmallenberg virus | Schmallenberg virus | Schmallenberg virus |
| MAR085_13 | Sabo virus | AF | Sabo virus | AF |
| MAR032_12 | Sango virus | AF | Shamonda virus | AF |
| KYA229_16 | Shuni virus | Shuni virus | AF | Shuni virus |
| GAU272_17 | Shamonda virus | AF | AF | Caimito virus |
| GAU110_14 | Shamonda virus | AF | AF | Caimito virus |
| MAR057_13 | Shuni virus | AF | AF | Caimito virus |
*AF, amplification failed; IS, insufficient sample; RT-PCR, reverse transcription PCR.
Page created: May 14, 2025
Page updated: June 18, 2025
Page reviewed: June 18, 2025
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