Volume 31, Number 7—July 2025
Dispatch
Extensively Drug-Resistant Neisseria gonorrhoeae Strain, Canada
Abstract
We identified a case of extensively drug-resistant (ceftriaxone- and cefixime-resistant and high-level azithromycin-resistant) Neisseria gonorrhoeae in Canada. The strain harbors the penA 60.001 allele, which includes the A311V and 23S rRNA A2059G single-nucleotide polymorphisms associated with high-level azithromycin resistance. The infection was likely acquired during travel in Cambodia.
Neisseria gonorrhoeae is the second most common sexually transmitted infection reported in Canada; the 92.34 cases/100,000 population reported 2022 represents an increase of 146.2% since 2010 (1). N. gonorrhoeae with third-generation cephalosporin resistance is in the high-priority category on the Bacterial Priority Pathogens List published by the World Health Organization in 2024 (https://iris.who.int/bitstream/handle/10665/376776/9789240093461-eng.pdf). Ceftriaxone is 1 of the last remaining empiric treatments options available for gonorrhea infection and is currently the recommended therapy in Canada. In 2017, we described a ceftriaxone-resistant N. gonorrhoeae isolate identified in North America (2). In 2018, we reported on the international dissemination of ceftriaxone-resistant N. gonorrhoeae isolates (3), identified in Japan as FC428. More than 10 extensively drug-resistant (XDR) N. gonorrhoeae isolates have been reported worldwide, most since 2022 (4). Those XDR isolates were found to harbor the penA-60.001 allele associated with ceftriaxone resistance and the 23S rRNA A2059G single-nucleotide polymorphism associated with high-level azithromycin resistance. We report an XDR N. gonorrhoeae isolate (no. 69155) identified in Canada that was cefixime and ceftriaxone resistant and has a high level of azithromycin resistance.
In May 2024, a man in his 40s experiencing urethritis sought care in an outpatient STI clinic in Montreal, Quebec, Canada. A strain of XDR N. gonorrhoeae from a urethral specimen was isolated at Centre Hospitalier de l’Université de Montréal. The patient reported that he had traveled to Cambodia and had sex with female sex workers there. He reported sexual contact with women only. He denied sexual contacts in Canada in the 60 days before the onset of his symptoms and had no sexual contacts upon his return. He was treated with 1 dose of oral azithromycin (2 g) combined with cefixime (800 mg) in accordance with treatment guidelines for sexually transmitted infections published in 2020 by Institut national d’excellence en santé et en services sociaux (INESSS) (https://www.bibliotheque.assnat.qc.ca/DepotNumerique_v2/AffichageNotice.aspx?idn=101746) but was still symptomatic at the time of the test of cure (TOC) by nucleic acid amplification tests (NAAT) 2 weeks later. We prescribed a second round of treatment with 1 intramuscular dose of ceftriaxone (250 mg) and oral doxycycline (100 mg 2×/d for 7 days); 2 weeks after the completion of the second treatment, the TOC by NAAT still remained positive. Because the patient was still symptomatic, providers used a syndromic approach; he was treated for clinical urethritis in accordance with the INESSS guidelines. The doxycycline was used to treat a possible C. trachomatis infection and not the N. gonorrhoeae infection. A third treatment of intramuscular ceftriaxone (500 mg) was successful; urethral culture and urine NAAT were negative at the third TOC.
We confirmed the bacterial isolate 69155 to be N. gonorrhoeae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (bioMérieux, https://www.biomerieux.com) and by whole-genome sequencing. We confirmed antimicrobial susceptibilities by agar dilution in accordance with Clinical and Laboratory Standards Institute (CLSI) guidance (5). The ceftriaxone MIC for this N. gonorrhoeae isolate was 0.25 mg/L, which is susceptible by CLSI guidelines and resistant by EUCAST guidelines (6). The cefixime MIC was 2 mg/L, which is nonsusceptible by CLSI guidelines and resistant by EUCAST guidelines. CLSI does not have a resistant breakpoint for cefixime or ceftriaxone; however, it defines MIC <0.25 mg/L as susceptible. EUCAST defines cefixime and ceftriaxone resistance as MIC >0.125 mg/L, whereas World Health Organization defines decreased susceptibility to cefixime as MIC >0.25 mg/L and to ceftriaxone as MIC >0.125 mg/L (7). Although there is no consistent resistance breakpoint, MICs of 0.25 mg/L for ceftriaxone and 2 mg/L for cefixime were previously reported in possible treatment failure cases (8,9). The isolate was also resistant to azithromycin (MIC >256 mg/L), ciprofloxacin (MIC 4 mg/L), tetracycline (MIC 32 mg/L), and penicillin (MIC >256 mg/L) but susceptible to spectinomycin (MIC 8 mg/L). We also determined MICs for gentamicin (MIC 4 mg/L) and ertapenem (MIC 0.5 mg/L).
We performed molecular typing in silico from whole-genome sequence data and submitted to the National Center for Biotechnology Information Sequence Read Archive (BioProject PRJNA415047, Sequence Read Archive no. SRR33123384). We sequenced the strain using Illumina NextSeq platform (Illumina, https://www.illumina.com) and used genomic quality, assembly, and annotation pipelines as previously described (10). We identified sequence type (ST) 16406 by multilocus sequence typing (MLST), ST-22862 by N. gonorrhoeae multiantigen sequence typing (NG-MAST; https://pubmlst.org/organisms/neisseria-spp), and ST-5793 by N. gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR; https://ngstar.canada.ca). The isolate had the mosaic penA-60.001 allele associated with ceftriaxone resistance and the A2059G single-nucleotide polymorphism in 23S rRNA associated with high-level azithromycin resistance. The penA-60.001 allele contains the A311V amino acid substitution, which is highly associated with ceftriaxone resistance (11). The MLST-determined ST16406 and penA-60.001 allele of the study isolate in Canada was identical to that of XDR N. gonorrhoeae strains isolated in Cambodia, Austria, United Kingdom, and France in 2022–2023 (4). Because only 1 strain from initial sampling was available, we did not perform genetic comparisons with other strains from this patient.
To compare the XDR N. gonorrhoeae strain we isolated with other ceftriaxone-resistant isolates, we performed phylogenetic analysis to determine single-nucleotide variants (SNV) using the custom Galaxy SNVPhyl20 pipeline (SNVPhyl version 1.0.1b Paired-End, https://github.com/phac-nml/snvphyl-galaxy) and included publicly available, internationally reported ceftriaxone-resistant and XDR gonorrhea genomes (BioProject PRJNA909328) (12) (Figure). The phylogenetic tree demonstrates the similarity of the Canada XDR–N. gonorrhoeae 69155 isolate to gonorrhea genomes reported in Southeast Asia; the study isolate differs from isolate SRR22570596 by 320 SNVs and SRR22570612 (BioProject PRJNA909328) by 140 SNVs.
We identified the bacterial isolate 69155 from a patient in Canada as a ceftriaxone-resistant and high-level azithromycin–resistant N. gonorrhoeae strain. The strain is of ST-16406 (MLST) and contains the penA-60.001 allele identical to cases previously reported in France, United Kingdom, Austria, and Cambodia. The patient’s infection was not cured by cefixime (800 mg) but by a higher dose of ceftriaxone (500 mg). Consistent with US Centers for Disease Control and Prevention recommendations (13), Quebec has recommended use of ceftriaxone (500 mg) as first-line treatment since September 2024 (14).
In 2020, guidelines in Europe recommended the use of ceftriaxone (1 g) as probabilistic treatment for uncomplicated genital infections (15). The European Centre for Disease Prevention and Control noted an increase of antimicrobial resistance in 2022 for N. gonorrhoeae (4). Moreover, >6 cases of XDR N. gonorrhoeae infection have been reported in Europe since 2022.
The surveillance of antimicrobial resistance in N. gonorrhoeae is crucial in the context of treatment failure and the dissemination of XDR strains worldwide. Our isolate was susceptible to ceftriaxone according to CLSI guidelines but resistant according to EUCAST guidelines; we therefore recommend using caution when interpreting borderline MICs. In Quebec, we published a directive asking frontline laboratories to promptly submit all third-generation cephalosporin borderline MIC isolates to the provincial laboratory for testing.
Dr. Lefebvre is the head of bacterial STIs at Laboratoire de santé publique du Québec. Her research centers on pathogens of major public health concern, including sexually transmitted infections such as Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Haemophilus ducreyi. She also studies vaccine-preventable pathogens including N. meningitidis, Streptococcus pneumoniae, H. influenzae, and Bordetella pertussis and other antibiotic-resistant pathogens, such as carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and methicillin-resistant Staphylococcus aureus.
Acknowledgment
Author contributions: B.L. and J.F. conceived and coordinated the study, performed experiments, analyzed data, and drafted the manuscript. I.M. and R.T. performed experiments and drafted the manuscript. A.B.-N., C.F., and J.G. coordinated the sample collection and/or provided clinical information about the case. All authors reviewed the final manuscript.
References
- Public Health Agency of Canada. Reported cases from 1924 to 2022 in Canada—notifiable diseases on-line. 2022 [cited 2025 Jan 6]. https://diseases.canada.ca/notifiable/charts?c=pl
- Lefebvre B, Martin I, Demczuk W, Deshaies L, Michaud S, Labbé AC, et al. Ceftriaxone-Resistant Neisseria gonorrhoeae, Canada, 2017. Emerg Infect Dis. 2018;24:381–3. DOIPubMedGoogle Scholar
- Lahra MM, Martin I, Demczuk W, Jennison AV, Lee K-I, Nakayama S-I, et al. Cooperative recognition of internationally disseminated ceftriaxone-resistant Neisseria gonorrhoeae strain. Emerg Infect Dis. 2018;24:735–40. DOIPubMedGoogle Scholar
- Caméléna F, Mérimèche M, Brousseau J, Mainardis M, Verger P, Le Risbé C, et al. Emergence of extensively drug-resistant Neisseria gonorrhoeae, France, 2023. Emerg Infect Dis. 2024;30:1903–6. DOIPubMedGoogle Scholar
- Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 34th edition. CLSI supplement M100. Wayne (PA): The Institute; 2024.
- European Committee on Antimicrobial Susceptibility Testing (EUCAST). Clinical breakpoints for bacteria. Version 14.0, 2024–01–01. 2024 [cited 2025 Jan 6]. https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_14.0_Breakpoint_Tables.pdf
- World Health Organization. Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. Geneva: The Organization; 2012 [cited 2025 Jan 6]. https://www.who.int/publications/i/item/9789241503501
- Pleininger S, Indra A, Golparian D, Heger F, Schindler S, Jacobsson S, et al. Extensively drug-resistant (XDR) Neisseria gonorrhoeae causing possible gonorrhoea treatment failure with ceftriaxone plus azithromycin in Austria, April 2022. Euro Surveill. 2022;27:
2200455 . DOIPubMedGoogle Scholar - Maubaret C, Caméléna F, Mrimèche M, Braille A, Liberge M, Mainardis M, et al. Two cases of extensively drug-resistant (XDR) Neisseria gonorrhoeae infection combining ceftriaxone-resistance and high-level azithromycin resistance, France, November 2022 and May 2023. Euro Surveill. 2023;28:
2300456 . DOIPubMedGoogle Scholar - Demczuk W, Lynch T, Martin I, Van Domselaar G, Graham M, Bharat A, et al. Whole-genome phylogenomic heterogeneity of Neisseria gonorrhoeae isolates with decreased cephalosporin susceptibility collected in Canada between 1989 and 2013. J Clin Microbiol. 2015;53:191–200. DOIPubMedGoogle Scholar
- Zhang L, Hu L, Li Y, Xiu L, Wang D, Huang J, et al. Identification of high-level ceftriaxone-resistant Neisseria gonorrhoeae isolates with diverse penA alleles in Zhejiang, China. J Glob Antimicrob Resist. 2023;35:51–5. DOIPubMedGoogle Scholar
- Ouk V, Say HL, Virak M, Deng S, Frankson R, McDonald R, et al.; EGASP Cambodia working group. World Health Organization Enhanced Gonococcal Antimicrobial Surveillance Programme, Cambodia, 2023. Emerg Infect Dis. 2024;30:1493–5. DOIPubMedGoogle Scholar
- Center for Diseases Control and Prevention. Sexually transmitted infections treatment guidelines: gonococcal infections among adolescents and adults. 2021 [cited 2025 Jan 6]. https://www.cdc.gov/std/treatment-guidelines/gonorrhea-adults.htm
- Institut national d’excellence en santé et en services sociaux. Pharmacological treatment guide on STIs—Chlamydia trachomatis infection and Neisseria gonorrhoeae infection [in French]. 2024 [cited 2025 Jan 6]. https://www.inesss.qc.ca/fileadmin/doc/INESSS/Outils/Guides_ITSS/Guide_ITSS-Chlamydia_gonorrhoeae.pdf
- Unemo M, Ross J, Serwin AB, Gomberg M, Cusini M, Jensen JS. 2020 European guideline for the diagnosis and treatment of gonorrhoea in adults. Int J STD AIDS. 2020;956462420949126:
956462420949126 . DOIPubMedGoogle Scholar
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Cite This ArticleOriginal Publication Date: June 20, 2025
Table of Contents – Volume 31, Number 7—July 2025
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Please use the form below to submit correspondence to the authors or contact them at the following address:
Brigitte Lefebvre, Laboratoire de santé publique du Québec, 20045 Chemin Ste-Marie, Ste-Anne-de-Bellevue, QB H9X 3R5, Canada
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