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Volume 31, Number 9—September 2025

Research

Insights into Infant Strongyloidiasis, Papua New Guinea

Huan Zhao, Juciliane Haidamak, Eva Noskova, Vladislav Ilik, Barbora Pafčo, Rebecca Ford, Geraldine Masiria, Tobias Maure, Nichola Kotale, William Pomat, Catherine Gordon, Severine Navarro, Paul F. Horwood, Constantin Constantinoiu, Andrew R. Greenhill, and Richard S. BradburyComments to Author 
Author affiliation: James Cook University, Townsville, Queensland, Australia (H. Zhao, J. Haidamak, P.F. Horwood, C. Constantinoiu, R.S. Bradbury); QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia (J. Haidamak, C. Gordon, S. Navarro); Czech Academy of Sciences, Brno, Czech Republic (E. Noskova, V. Ilik, B. Pafčo); Masaryk University, Brno (E. Noskova, V. Ilik, B. Pafčo); Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea (R. Ford, G. Masiria, T. Maure, N. Kotale, W. Pomat); University of Queensland, Brisbane (C. Gordon); Federation University Australia, Gippsland, Victoria, Australia (A.R. Greenhill).

Main Article

Figure 3

Euler diagram showing the performance of 2 qPCRs (20,21) and 18S rRNA HVR-IV metabarcoding (16) for the detection of Strongyloides spp. in 164 infant fecal samples from Papua New Guinea. Values in parentheses are no. positive samples/total no. tested. HVR, hypervariable region; qPCR, quantitative PCR.

Figure 3. Euler diagram showing the performance of 2 qPCRs (20,21) and 18S rRNA HVR-IV metabarcoding (16) for the detection of Strongyloides spp. in 164 infant fecal samples from Papua New Guinea. Values in parentheses are no. positive samples/total no. tested. HVR, hypervariable region; qPCR, quantitative PCR.

Main Article

References
  1. Bradbury  RS. Strongyloides fuelleborni kellyi in New Guinea: neglected, ignored and unexplored. Microbiol Aust. 2021;42:169–72. DOIGoogle Scholar
  2. Page  W, Shield  J, O’Donahoo  F, Miller  A, Judd  J, Speare  R. Strongyloidiasis in Oceania. In: Hotez PT, editor. Neglected tropical diseases-Oceania. Switzerland: Springer; 2016. p. 69–99.
  3. Viney  M, Ashford  R, Barnish  G. A taxonomic study of Strongyloides Grassi, 1879 (nematoda) with special reference to Strongyloides fuelleborni von Linstow, 1905 in man in Papua New Guinea and the description of a new subspecies. Syst Parasitol. 1991;18:95–109. DOIGoogle Scholar
  4. Kelly  A, Voge  M. Report of a nematode found in humans at Kiunga, Western District. P N G Med J. 1973;16:59.
  5. Muller  R, Lillywhite  J, Bending  JJ, Catford  JC. Human cysticercosis and intestinal parasitism amongst the Ekari people of Irian Jaya. J Trop Med Hyg. 1987;90:291–6.PubMedGoogle Scholar
  6. Ashford  RW, Barnish  G, Viney  ME. Strongyloides fuelleborni kellyi: infection and disease in Papua New Guinea. Parasitol Today. 1992;8:314–8. DOIPubMedGoogle Scholar
  7. Viney  ME, Ashford  RW. The use of isoenzyme electrophoresis in the taxonomy of Strongyloides. Ann Trop Med Parasitol. 1990;84:35–47. DOIPubMedGoogle Scholar
  8. Barnish  G, Ashford  RW. Strongyloides cf fuelleborni in Papua New Guinea: epidemiology in an isolated community, and results of an intervention study. Ann Trop Med Parasitol. 1989;83:499–506. DOIPubMedGoogle Scholar
  9. Ashford  RW, Vince  JD, Gratten  MJ, Bana-Koiri  J. Strongyloides infection in a mid-mountain Papua New Guinea community: results of an epidemiological survey. 1979. P N G Med J. 2005;48:58–65.PubMedGoogle Scholar
  10. Vince  JD, Ashford  RW, Gratten  MJ, Bana-Koiri  J. Strongyloides species infestation in young infants of Papua New Guinea: association with generalized oedema. 1979. P N G Med J. 2005;48:50–7.PubMedGoogle Scholar
  11. Barnish  G, Harari  M. Possible effects of Strongyloides fuelleborni-like infections on children in the Karimui area of Simbu Province. P N G Med J. 1989;32:51–4.PubMedGoogle Scholar
  12. King  SE, Mascie-Taylor  CGS. Strongyloides fuelleborni kellyi and other intestinal helminths in children from Papua New Guinea: associations with nutritional status and socioeconomic factors. P N G Med J. 2004;47:181–91.PubMedGoogle Scholar
  13. Dorris  M, Viney  ME, Blaxter  ML. Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. Int J Parasitol. 2002;32:1507–17. DOIPubMedGoogle Scholar
  14. Hasegawa  H, Hayashida  S, Ikeda  Y, Sato  H. Hyper-variable regions in 18S rDNA of Strongyloides spp. as markers for species-specific diagnosis. Parasitol Res. 2009;104:869–74. DOIPubMedGoogle Scholar
  15. Aupalee  K, Wijit  A, Singphai  K, Rödelsperger  C, Zhou  S, Saeung  A, et al. Genomic studies on Strongyloides stercoralis in northern and western Thailand. Parasit Vectors. 2020;13:250. DOIPubMedGoogle Scholar
  16. Barratt  JLN, Lane  M, Talundzic  E, Richins  T, Robertson  G, Formenti  F, et al. A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing. PLoS Negl Trop Dis. 2019;13:e0007609. DOIPubMedGoogle Scholar
  17. Hino  A, Tanaka  T, Takaishi  M, Fujii  Y, Palomares-Rius  JE, Hasegawa  K, et al. Karyotype and reproduction mode of the rodent parasite Strongyloides venezuelensis. Parasitology. 2014;141:1736–45. DOIPubMedGoogle Scholar
  18. Ko  PP, Haraguchi  M, Hara  T, Hieu  DD, Ito  A, Tanaka  R, et al. Population genetics study of Strongyloides fuelleborni and phylogenetic considerations on primate-infecting species of Strongyloides based on their mitochondrial genome sequences. Parasitol Int. 2023;92:102663. DOIPubMedGoogle Scholar
  19. de Ree  V, Nath  TC, Barua  P, Harbecke  D, Lee  D, Rödelsperger  C, et al. Genomic analysis of Strongyloides stercoralis and Strongyloides fuelleborni in Bangladesh. PLoS Negl Trop Dis. 2024;18:e0012440. DOIPubMedGoogle Scholar
  20. Llewellyn  S, Inpankaew  T, Nery  SV, Gray  DJ, Verweij  JJ, Clements  AC, et al. Application of a multiplex quantitative PCR to assess prevalence and intensity of intestinal parasite infections in a controlled clinical trial. PLoS Negl Trop Dis. 2016;10:e0004380. DOIPubMedGoogle Scholar
  21. Pilotte  N, Papaiakovou  M, Grant  JR, Bierwert  LA, Llewellyn  S, McCarthy  JS, et al. Improved PCR-based detection of soil transmitted helminth infections using a next-generation sequencing approach to assay design. PLoS Negl Trop Dis. 2016;10:e0004578. DOIPubMedGoogle Scholar
  22. Jiang  H, Lei  R, Ding  S-W, Zhu  S. Skewer: a fast and accurate adapter trimmer for next-generation sequencing paired-end reads. BMC Bioinformatics. 2014;15:182. DOIPubMedGoogle Scholar
  23. Callahan  BJ, McMurdie  PJ, Rosen  MJ, Han  AW, Johnson  AJA, Holmes  SP. DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016;13:581–3. DOIPubMedGoogle Scholar
  24. Janwan  P, Rodpai  R, Intapan  PM, Sanpool  O, Tourtip  S, Maleewong  W, et al. Possible transmission of Strongyloides fuelleborni between working Southern pig-tailed macaques (Macaca nemestrina) and their owners in Southern Thailand: Molecular identification and diversity. Infect Genet Evol. 2020;85:104516. DOIPubMedGoogle Scholar
  25. Pampiglione  S, Ricciardi  M. Geographic distribution of Strongyloides fulleborni in humans in tropical Africa. Parasitologia. 1972;14:329–38.
  26. Hira  PR, Patel  BG. Human strongyloidiasis due to the primate species Strongyloides fülleborni. Trop Geogr Med. 1980;32:23–9.PubMedGoogle Scholar
  27. Barratt  JLN, Sapp  SGH. Machine learning-based analyses support the existence of species complexes for Strongyloides fuelleborni and Strongyloides stercoralis. Parasitology. 2020;147:1184–95. DOIPubMedGoogle Scholar
  28. Kelly  A, Little  MD, Voge  M. Strongyloides fulleborni-like infections in man in Papua New Guinea. Am J Trop Med Hyg. 1976;25:694–9. DOIPubMedGoogle Scholar
  29. Barnish  G, Ashford  RW. Strongyloides cf. fuelleborni and hookworm in Papua New Guinea: patterns of infection within the community. Trans R Soc Trop Med Hyg. 1989;83:684–8. DOIPubMedGoogle Scholar
  30. Brown  RC, Girardeau  HF. Transmammary passage of Strongyloides sp. larvae in the human host. Am J Trop Med Hyg. 1977;26:215–9. DOIPubMedGoogle Scholar
  31. Constable  P, Hinchcliff  K, Done  S, Grünberg  W. Diseases of the alimentary tract: nonruminant. Veterinary medicine. 11th ed. St. Louis: Elsevier; 2017.
  32. Uzal  FA, Plattner  BL, Hostetter  JM. Alimentary system. In: Maxie M, editor. Jubb, Kennedy & Palmer’s pathology of domestic animals. 6th ed. Philadelphia: W.B. Saunders; 2016. p. 1–257.e2.
  33. Dey-Hazra  A, Sallmann  HP, Enigk  K, Harisch  G. Protein synthesis changes in the liver of piglets infected with Strongyloides ransomi. Vet Parasitol. 1979;5:339–51. DOIGoogle Scholar
  34. Verweij  JJ, Canales  M, Polman  K, Ziem  J, Brienen  EA, Polderman  AM, et al. Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Trans R Soc Trop Med Hyg. 2009;103:342–6. DOIPubMedGoogle Scholar

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