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Volume 32, Number 1—January 2026

Dispatch

Detection of Avian Influenza H5–Specific Antibodies by Chemiluminescent Assays

Ana Citlali Márquez, Saina Beitari, Tahereh Valadbeigy, Linda Hoang, Yohannes Berhane, and Agatha N. JassemComments to Author 
Author affiliation: British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada (A.C. Márquez, S. Beitari, T. Valadbeigy, L. Hoang, A.N. Jassem); University of British Columbia, Vancouver (A.C. Márquez, L. Hoang, A.N. Jassem); Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada (Y. Berhane); University of Manitoba, Winnipeg (Y. Berhane); University of Saskatchewan, Saskatoon, Saskatchewan, Canada (Y. Berhane)

Main Article

Figure

Specific detection of H5 antibodies in human and avian samples in a study on detection of avian influenza H5–specific antibodies by chemiluminescent assays. A) Results of human serum sample testing by using the MSD V-Plex Respiratory Panel 7 (IgG) kit (MSD, https://www.mesoscale.com), diluted 1:5,000 in the manufacturer-provided diluent. B) Results of human serum sample testing by using the MSD Influenza H5 Bridging Serology Assay (MSD, https://www.mesoscale.com), diluted 1:20 and prepared according to the manufacturer’s instructions. C) Results of dried blood spot sample testing by using the MSD Influenza H5 Bridging Serology Assay at 1:10 dilution. D) H5 antibody polyclonal and avian sample results obtained from chickens inoculated with various influenza subtypes and tested on the MSD Influenza H5 Bridging Serology Assay according to the manufacturer's instructions. Antibody titer production was confirmed by hemagglutinin inhibition assay. *A/chicken/Vietnam/14/2005; †H5 DNA vaccinated with HA from A/mallard/BC/373/2005 (H5N2) and challenged with A/chicken/Vietnam/14/2005 (H5N1); ‡A/teal/Germany/Wv632/2005; AU, arbitrary units; MSD, Meso Scale Discovery.

Figure. Specific detection of H5 antibodies in human and avian samples in a study on detection of avian influenza H5–specific antibodies by chemiluminescent assays. A) Results of human serum sample testing by using the MSD V-Plex Respiratory Panel 7 (IgG) kit (MSD, https://www.mesoscale.com), diluted 1:5,000 in the manufacturer-provided diluent. B) Results of human serum sample testing by using the MSD Influenza H5 Bridging Serology Assay (MSD, https://www.mesoscale.com), diluted 1:20 and prepared according to the manufacturer’s instructions. C) Results of dried blood spot sample testing by using the MSD Influenza H5 Bridging Serology Assay at 1:10 dilution. D) H5 antibody polyclonal and avian sample results obtained from chickens inoculated with various influenza subtypes and tested on the MSD Influenza H5 Bridging Serology Assay according to the manufacturer's instructions. Antibody titer production was confirmed by hemagglutinin inhibition assay. *A/chicken/Vietnam/14/2005; †H5 DNA vaccinated with HA from A/mallard/BC/373/2005 (H5N2) and challenged with A/chicken/Vietnam/14/2005 (H5N1); ‡A/teal/Germany/Wv632/2005; AU, arbitrary units; MSD, Meso Scale Discovery.

Main Article

Page created: December 30, 2025
Page updated: January 29, 2026
Page reviewed: January 29, 2026
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