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Volume 32, Number 1—January 2026

Dispatch

Evidence of Rat Hepatitis E Virus Circulation through Wastewater Surveillance, Central Argentina

Bianca Filoni1Comments to Author , María Emilia Lucero1, Guadalupe Di Cola, Anabella Fantilli, Alfonsina Roccia, Paola Sicilia, Liliana Luque, Ariana Cachi, María de los Ángeles Marinzalda, Gonzalo Castro, Gisela Masachessi, Viviana Ré, and María Belén Pisano
Author affiliation: Instituto de Virología Dr. J.M. Vanella, Universidad Nacional de Córdoba, Córdoba, Argentina (B. Filoni, M.E. Lucero, G. Di Cola, A. Fantilli, A. Roccia, L. Luque, A. Cachi, M. de los Ángeles Marinzalda, G. Castro, G. Masachessi, V. Ré, M.B. Pisano); Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina (G. Di Cola, G. Masachessi, V. Ré, M.B. Pisano); Ministerio de Salud de la Provincia de Córdoba, Córdoba (P. Sicilia, L. Luque, G. Castro); Instituto Nacional de Medicina Aeronáutica y Espacial, Facultad de la Fuerza Aérea, Facultad de la Fuerza Aérea, Universidad de la Defensa Nacional, Córdoba (A. Cachi, M. de los Ángeles Marinzalda).

Main Article

Figure 2

Maximum-likelihood phylogenetic tree on the basis of a 338 bp fragment of the open reading frame 1 genomic region of rat hepatitis E virus (R-HEV; Rocahepevirus ratti) from Argentina, 2023–2024. Red text indicates sequences obtained during this study. The tree includes representative sequences of each R-HEV genotype available in GenBank database, additional sequences of proposed clades (clade I in blue, clade II in pink) and subtypes a (square), b (circle), c (5-pointed star), d (4-pointed star), e (rhombus), and f (triangle) (13). Two hepatitis E virus (Paslahepevirus balayani) sequences (genotypes 1 and 3) were used as outgroups. Enlarged area shows sequences from this study and the first 10 most similar sequences from BLAST analysis (https://blast.ncbi.nlm.nih.gov). Statistical support values are indicated at nodes; only supports over 70/70 are shown. Scale bar represents the number of substitutions per site.

Figure 2. Maximum-likelihood phylogenetic tree on the basis of a 338 bp fragment of the open reading frame 1 genomic region of rat hepatitis E virus (R-HEV; Rocahepevirus ratti) from Argentina, 2023–2024. Red text indicates sequences obtained during this study. The tree includes representative sequences of each R-HEV genotype available in GenBank database, additional sequences of proposed clades (clade I in blue, clade II in pink) and subtypes a (square), b (circle), c (5-pointed star), d (4-pointed star), e (rhombus), and f (triangle) (13). Two hepatitis E virus (Paslahepevirus balayani) sequences (genotypes 1 and 3) were used as outgroups. Enlarged area shows sequences from this study and the first 10 most similar sequences from BLAST analysis (https://blast.ncbi.nlm.nih.gov). Statistical support values are indicated at nodes; only supports over 70/70 are shown. Scale bar represents the number of substitutions per site.

Main Article

References
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1These authors contributed equally to this article.

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