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Wastewater Surveillance to Track Resurgent Measles Outbreak, Ontario, Canada, 2025
Ryland Corchis-Scott

, Élisabeth Mercier, Edgard M. Mejia, Qiudi Geng, Ethan Harrop, Ana Podadera, Jackie Fong, Natalie Lewoc, Kenneth K.S. Ng, Nataliya Santiago, Natalie Knox, Lawrence Goodridge, Chand S. Mangat, Chrystal Landgraff, Karen B. Riddell, Mehdi Aloosh, Robert Delatolla, and R. Michael McKay
Author affiliation: University of Windsor, Windsor, Ontario, Canada (R. Corchis-Scott, Q. Geng, E. Harrop, A. Podadera, J. Fong, K.K.S. Ng, R.M. McKay); University of Ottawa, Ottawa, Ontario, Canada (É. Mercier, R. Delatolla); National Microbiology Laboratory, Winnipeg, Manitoba, Canada (E.M. Mejia, N. Santiago, N. Knox, C.S. Mangat, C. Landgraff); Windsor Essex County Health Unit, Windsor (N. Lewoc, M. Aloosh); University of Guelph, Guelph, Ontario, Canada (L. Goodridge); Windsor Regional Hospital, Windsor (K.B. Riddell); McMaster University, Hamilton, Ontario, Canada (M. Aloosh)
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Figure 2

Figure 2. Partitioning of MeV signal across wastewater fractions in a study of wastewater surveillance conducted in Leamington, Ontario, Canada, during the 2025 outbreak. Partitioning of measles virus is shown for 3 calculation bases. Each point represents 1 technical triplicate for each of the biologic replicates (n = 14). For each bar, the upper border of each bar indicates the mean; whiskers indicate standard error. Where the standard deviation is too small, the error bars are not displayed. A) Signal partitioned assuming the composition of a typical wastewater sample (99.96% liquid, 0.04% solids). B) Signal partitioned on an equivalent mass basis, assuming 1 g of each fraction. C) Signal partitioned based on typical laboratory processing volumes: 0.25 g for settled solids, 150 mL for the colloids fraction, and 37 mL for the supernatant. MeV, measles virus.
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