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Volume 32, Number 11—November 2026
Dispatch
Dirofilaria asiatica Infection and Asymptomatic Microfilaremia, Sri Lanka, 2025
Table
Characteristics of Dirofilaria microfilariae detected in study of Dirofilaria asiatica infection and asymptomatic microfilaremia, compared with those of reference strains, Sri Lanka, 2025*
| Morphology | Microfilaria in this study | D. repens microfilaria† | D. asiatica microfilaria‡ |
|---|---|---|---|
| Sheath | Absent | Absent | Absent |
| Mean total body length, µm | 349 | 369 +10 | 300.3 |
| Mean width, µm |
8 |
8.87+0.5 |
5.7 |
| Cephalic space | |||
| Mean length, µm | 2.5 | 2.96 + 1.18 | 3.27 |
| Location | Round anterior end | Round anterior end | Not specified |
| No. nuclei |
2–3 |
2 nuclei |
2–3 nuclei |
| Tail | Tapering, curved, free of nuclei | Tapering, curved, free of nuclei | Tapering, curved, free of nuclei |
*Specimens in this study were human blood smears stained with 10% Giemsa; we observed a mean of 10 microfilariae per specimen. Characteristics were obtained using Olympus microscope (original magnification ×1,000) and cell analysis software ScopeImage version 9.0 (https://www.bioimager.com). †Magnis et al. (5). ‡Colella et al. (2) Specimens were stained with 0.1% methylene blue.
References
- Simón F, Siles-Lucas M, Morchón R, González-Miguel J, Mellado I, Carretón E, et al. Human and animal dirofilariasis: the emergence of a zoonotic mosaic. Clin Microbiol Rev. 2012;25:507–44. DOIPubMedGoogle Scholar
- Colella V, Young ND, Manzanell R, Atapattu U, Sumanam SB, Huggins LG, et al. Dirofilaria asiatica sp. nov. (Spirurida: Onchocercidae)—defined using a combined morphological-molecular approach. Int J Parasitol. 2025;55:461–74. DOIPubMedGoogle Scholar
- Atapattu U, Koehler AV, Huggins LG, Wiethoelter A, Traub RJ, Colella V. Dogs are reservoir hosts of the zoonotic Dirofilaria sp. ‘hongkongensis’ and potentially of Brugia sp. Sri Lanka genotype in Sri Lanka. One Health. 2023;17:
100625 . DOIPubMedGoogle Scholar - Pampiglione S, Rivasi F. Human dirofilariasis due to Dirofilaria (Nochtiella) repens: an update of world literature from 1995 to 2000. Parassitologia. 2000;42:231–54.PubMedGoogle Scholar
- Magnis J, Lorentz S, Guardone L, Grimm F, Magi M, Naucke TJ, et al. Morphometric analyses of canine blood microfilariae isolated by the Knott’s test enables Dirofilaria immitis and D. repens species-specific and Acanthocheilonema (syn. Dipetalonema) genus-specific diagnosis. Parasit Vectors. 2013;6:48. DOIPubMedGoogle Scholar
- Balendran T, Yatawara L, Wickramasinghe S. Human dirofilariasis caused by Dirofilaria repens in Sri Lanka from 1962 to 2020. Acta Parasitol. 2022;67:628–39. DOIPubMedGoogle Scholar
- Poppert S, Hodapp M, Krueger A, Hegasy G, Niesen WD, Kern WV, et al. Dirofilaria repens infection and concomitant meningoencephalitis. Emerg Infect Dis. 2009;15:1844–6. DOIPubMedGoogle Scholar
- Frenzen FS, Loewe I, Müller G, Schoenlebe J, Tappe D, Teichmann D. Dirofilaria repens infection of the eye with concomitant microfilaremia in a traveller. J Travel Med. 2021;28:
taaa119 . DOIPubMedGoogle Scholar - Casiraghi M, Anderson TJ, Bandi C, Bazzocchi C, Genchi C. A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology. 2001;122:93–103. DOIPubMedGoogle Scholar
- Tamura K, Stecher G, Kumar S. MEGA11: Molecular Evolutionary Genetics Analysis version 11. Mol Biol Evol. 2021;38:3022–7. DOIPubMedGoogle Scholar
- Valčiukaitė-Žilinskienė R, Zablockienė B, Zablockis R. Dirofilariasis presenting as pleural effusion: a rare case report with unusual manifestations and treatment modalities. BMC Pulm Med. 2024;24:340. DOIPubMedGoogle Scholar
- Biasizzo H, Šoba B, Ilovski F, Harlander M, Lukin M, Blatnik O, et al. Severe and rare case of human Dirofilaria repens infection with pleural and subcutaneous manifestations, Slovenia. Emerg Infect Dis. 2022;28:2504–7. DOIPubMedGoogle Scholar
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