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Volume 32, Number 7—July 2026

Dispatch

Nipah Virus Shedding in Urine from Fruit Bats, Sri Lanka, 2018–2019

Claudia Kohl1Comments to Author , Sahan Siriwardana1, Therese Muzeniek, Thejanee Perera, Dilara Bas, Mizgin Öruc, Annika Brinkmann, Beate Becker-Ziaja, Franziska Schwarz, Hamsananthy Jeevatharan, Jagathpriya Weerasena, Shiroma Handunnetti, Inoka C. Perera, Gayani Premawansa, Sunil Premawansa, Wipula Yapa, and Andreas Nitsche
Author affiliation: Robert Koch Institute, Berlin, Germany (C. Kohl, T. Muzeniek, T. Perera, D. Bas, M. Öruc, A. Brinkmann, B. Becker-Ziaja. F. Schwarz, A. Nitsche); University of Colombo, Columbo, Sri Lanka (S. Siriwardana, T. Perera, J. Weerasena, S. Handunetti, I.C. Perera, S. Premawansa, W. Yapa); Ministry of Health, Colombo (H. Jeevatharan); Colombo North Teaching Hospital, Ragama, Sri Lanka (G. Premawansa)

Main Article

Table 1

Primers and cycling conditions for quantitative PCR assay developed for study of NiV shedding in urine from fruit bats, Sri Lanka, 2018–2019

Component
 Conditions
Primers
NiV_F AAA TCA AGT TGC AGA ACT CGC T
NiV_R CTC CRA TGA GCA CAC CTC CTG
NiV Probe
 FAM-CTT CCT GCT GAT GTT TC- MGB
Protocol†
 AgPath-ID One-Step RT-PCR Kit,‡ 6 µL H20, 12.5 µL RT buffer, 1 µL NiV_F (10 µM), 1 µL NiV_R (10 µM), 0.5 µL NiVN_probe (10 µM), 1.0 µL enzyme mix (25×)
Cycling conditions
 45°C for 900 s, 95°C for 600 s, 95°C for 15 s for 45 cycles, 60°C for 45 s
Cycler types Light Cycler 480,§ ABI 7500,‡ Bio-Rad CFX 96¶

*F, forward primer; FAM, fluorescein amidites; MGB, minor groove binder; NiV, Nipah virus; R, reverse primer. †The primers listed are all included in the mix. ‡Applied Biosystems, https://www.thermofisher.com. §Roche Life Science, https://lifescience.roche.com. ¶Bio-Rad Laboratories, https://www.bio-rad.com.

Main Article

1These first authors contributed equally to this article.

Page created: April 13, 2026
Page updated: June 26, 2026
Page reviewed: June 26, 2026
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