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Volume 32, Number 8—August 2026

Dispatch

Detection of Congenital Syphilis via Digital PCR and Next-Generation Sequencing, Colombia

Ana M. Bossa-Castro, Narda M. Olarte EscobarComments to Author , Ismael Alberto Valderrama Márquez, Deisy Abril, Luis Fuentes, Zayda Lorena Corredor-Rozo, Ruth Liliana López Cruz, Sharon Hassbleidy Ochoa Ramírez, José Alejandro Mojica Madera, Pedro José Ramos Cabrera, Jorge Eliecer Castellanos Corredor, Nidia Aydee Garay Bernal, Ana María Gómez Puentes, Carlos Alberto Morales Pertuz, and Javier Escobar-PérezComments to Author 
Author affiliation: Universidad El Bosque, Vicerrectoría de Investigaciones, Bacterial Molecular Genetics Laboratory, Bogota, Colombia (A.M. Bossa-Castro, D. Abril, L. Fuentes, Z.L. Corredor-Rozo, A.M. Gómez Puentes, J. Escobar-Pérez); Subred Integrada de Servicios de Salud Sur E.S.E., Bogota (N.M. Olarte Escobar, I.A. Valderrama Márquez, R.L. López Cruz, S.H. Ochoa Ramírez, J.A. Mojica Madera, P.J. Ramos Cabrera, J.E. Castellanos Corredor, N.A. Garay Bernal, C.A. Morales Pertuz)

Main Article

Figure

Comparison of testing results for detection of congenital syphilis via dPCR and NGS, Colombia. The Treponema pallidum tp0547 gene concentration was calculated by the dPCR platform software, and NGS sequencing was performed on the MinION Mk1B (Oxford Nanopore Technologies, https://www.nanoporetech.com). The VDRL results at each time of sampling and number of confirmed penicillin doses in the mother are listed below the graph. CSF, cerebrospinal fluid; dPCR, digital PCR; NGS, next-generation sequencing; NR, not reactive; VDRL, venereal disease research laboratory.

Figure. Comparison of testing results for detection of congenital syphilis via dPCR and NGS, Colombia. The Treponema pallidum tp0547 gene concentration was calculated by the dPCR platform software, and NGS sequencing was performed on the MinION Mk1B (Oxford Nanopore Technologies, https://www.nanoporetech.com). The VDRL results at each time of sampling and number of confirmed penicillin doses in the mother are listed below the graph. CSF, cerebrospinal fluid; dPCR, digital PCR; NGS, next-generation sequencing; NR, not reactive; VDRL, venereal disease research laboratory.

Main Article

Page created: June 23, 2026
Page updated: July 17, 2026
Page reviewed: July 17, 2026
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