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Volume 32, Number 8—August 2026

Dispatch

Filovirus Surveillance in Communities Bordering Equatorial Guinea, Marburg Outbreak, Cameroon, 2023

Jill-Léa Ramassamy, Flaubert Auguste Mba Djondzo1, Innocent Ndong Bass, Ginette Edoul, Dowbiss Meta-Djomsi, Nadine Lamare, Maeliss Champagne, Célestin Godwe, Kono Léon, Cavour Tadjouteu, Audrey Lacroix, Nicole Vidal, Guillaume Thaurignac, Elisabeth Dibongue, Eric Delaporte, Martine Peeters, Ahidjo Ayouba2, and Charles Kouanfack2
Author affiliation: Université de Montpellier, IRD, INSERM, Montpellier, France (J.-L. Ramassamy, M. Champagne, A. Lacroix, N. Vidal, G. Thaurignac, E. Delaporte, M. Peeters, A. Ayouba); Centre de Recherche sur les Maladies Emergentes et Réémergentes (CREMER), Yaoundé, Cameroon (F.A.M. Djondzo, I.N. Bass, G. Edoul, D. Meta-Djomsi, N. Lamare, C. Godwe, A. Ayouba, C. Kouanfack); University of Yaounde, Yaoundé (K. Léon); Yaoundé Central Hospital, Yaoundé (C. Tadjouteu); Cameroon National OneHealth Platform, Yaoundé (E. Dibongue); University of Dschang, Dschang, Cameroon (C. Kounanfack)

Main Article

Figure 1

Study area of One Health surveillance for MARV in communities bordering Equatorial Guinea Marburg outbreak, Cameroon, 2023. A) Locations in Equatorial Guinea with confirmed and probable MVD cases during the outbreak (5) and study area in Cameroon (black box) with integrated surveillance sites (triangle, circle, and square icons). Inset map shows location of study area in Africa. B) Human survey villages; circle size indicates number of participants (n = 181 total participants). Stars indicate villages with MARV seropositive participants, defined as reactivity to >2 antigens (1.7%, n = 3 participants). C) Bat capture sites with species composition (n = 288 bats). Rousettus aegyptiacus bats, the MARV reservoir species, were most abundant (54.9%, n = 158 bats). We did not detect MARV RNA by using panfilovirus reverse transcription PCR of 589 bat samples. MARV, Marburg virus; MVD, Marburg virus disease.

Figure 1. Study area of One Health surveillance for MARV in communities bordering Equatorial Guinea Marburg outbreak, Cameroon, 2023. A) Locations in Equatorial Guinea with confirmed and probable MVD cases during the outbreak (5) and study area in Cameroon (black box) with integrated surveillance sites (triangle, circle, and square icons). Inset map shows location of study area in Africa. B) Human survey villages; circle size indicates number of participants (n = 181 total participants). Stars indicate villages with MARV seropositive participants, defined as reactivity to >2 antigens (1.7%, n = 3 participants). C) Bat capture sites with species composition (n = 288 bats). Rousettus aegyptiacus bats, the MARV reservoir species, were most abundant (54.9%, n = 158 bats). We did not detect MARV RNA by using panfilovirus reverse transcription PCR of 589 bat samples. MARV, Marburg virus; MVD, Marburg virus disease.

Main Article

References
  1. Cuomo-Dannenburg  G, McCain  K, McCabe  R, Unwin  HJT, Doohan  P, Nash  RK, et al.; Pathogen Epidemiology Review Group. Marburg virus disease outbreaks, mathematical models, and disease parameters: a systematic review. Lancet Infect Dis. 2024;24:e30717. DOIPubMedGoogle Scholar
  2. Towner  JS, Pourrut  X, Albariño  CG, Nkogue  CN, Bird  BH, Grard  G, et al. Marburg virus infection detected in a common African bat. PLoS One. 2007;2:e764. DOIPubMedGoogle Scholar
  3. Bausch  DG, Borchert  M, Grein  T, Roth  C, Swanepoel  R, Libande  ML, et al. Risk factors for Marburg hemorrhagic fever, Democratic Republic of the Congo. Emerg Infect Dis. 2003;9:15317. DOIPubMedGoogle Scholar
  4. Towner  JS, Amman  BR, Sealy  TK, Carroll  SAR, Comer  JA, Kemp  A, et al. Isolation of genetically diverse Marburg viruses from Egyptian fruit bats. PLoS Pathog. 2009;5:e1000536. DOIPubMedGoogle Scholar
  5. Ngai  S, Evers  ES, Seoane  AKL, Ameh  G, Anoko  JN, Barnadas  C, et al.; Marburg Virus Disease Outbreak Response Working Group. Outbreak of Marburg virus disease, Equatorial Guinea, 2023. Emerg Infect Dis. 2025;31:88795. DOIPubMedGoogle Scholar
  6. Harris  E. WHO: Marburg virus outbreak confirmed in Equatorial Guinea. JAMA. 2023;329:969. DOIPubMedGoogle Scholar
  7. Ayouba  A, Touré  A, Butel  C, Keita  AK, Binetruy  F, Sow  MS, et al. Development of a sensitive and specific serological assay based on Luminex technology for detection of antibodies to Zaire Ebola virus. J Clin Microbiol. 2016;55:16576. DOIPubMedGoogle Scholar
  8. Goldstein  T, Anthony  SJ, Gbakima  A, Bird  BH, Bangura  J, Tremeau-Bravard  A, et al. The discovery of Bombali virus adds further support for bats as hosts of ebolaviruses. Nat Microbiol. 2018;3:10849. DOIPubMedGoogle Scholar
  9. De Nys  HM, Kingebeni  PM, Keita  AK, Butel  C, Thaurignac  G, Villabona-Arenas  CJ, et al. Survey of Ebola viruses in frugivorous and insectivorous bats in Guinea, Cameroon, and the Democratic Republic of the Congo, 2015–2017. Emerg Infect Dis. 2018;24:222840. DOIPubMedGoogle Scholar
  10. Ramassamy  JL, Ayouba  A, Thaurignac  G, Bilounga Ndongo  C, Nnuka  P, Betsem  E, et al. High seroreactivities to Orthoebolaviruses in rural Cameroon: a case-control study on nonhuman primate bites and a cross-sectional survey in rural populations. J Infect Dis. 2024;230:e106776. DOIPubMedGoogle Scholar
  11. Amman  BR, Schuh  AJ, Albariño  CG, Towner  JS. Marburg virus persistence on fruit as a plausible route of bat to primate filovirus transmission. Viruses. 2021;13:2394. DOIPubMedGoogle Scholar
  12. Baudel  H, De Nys  H, Mpoudi Ngole  E, Peeters  M, Desclaux  A. Understanding Ebola virus and other zoonotic transmission risks through human–bat contacts: exploratory study on knowledge, attitudes and practices in Southern Cameroon. Zoonoses Public Health. 2019;66:28895. DOIPubMedGoogle Scholar
  13. Ramassamy  JL, Ndongo  CB, Nnuka  P, Njouom  R, Gessain  A. High potential for zoonotic pathogen emergence through wildlife exposure in East Cameroon. One Health. 2025;21:101275. DOIPubMedGoogle Scholar
  14. Amman  BR, Carroll  SA, Reed  ZD, Sealy  TK, Balinandi  S, Swanepoel  R, et al. Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human infection. PLoS Pathog. 2012;8:e1002877. DOIPubMedGoogle Scholar
  15. Makenov  MT, Boumbaly  S, Tolno  FR, Sacko  N, N’Fatoma  LT, Mansare  O, et al. Marburg virus in Egyptian Rousettus bats in Guinea: investigation of Marburg virus outbreak origin in 2021. PLoS Negl Trop Dis. 2023;17:e0011279. DOIPubMedGoogle Scholar

Main Article

1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

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