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Volume 6, Number 5—October 2000

Imported Lassa Fever in Germany: 
Molecular Characterization of a New Lassa Virus Strain

Stephan GüntherComments to Author , Petra Emmerich, Thomas Laue, Olaf Kühle, Marcel Asper, Annegret Jung, Thomas Grewing, Jan ter Meulen, and Herbert Schmitz

Author affiliations: Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

Article in Chinese

Main Article

Figure 1

Map of West Africa and travel history of the patient before the onset of febrile illness (day 0). Countries in which Lassa fever is endemic (1-4) are underlined.

Figure 1. Map of West Africa and travel history of the patient before the onset of febrile illness (day 0). Countries in which Lassa fever is endemic (1-4) are underlined.

Main Article

  1. Carey  DE, Kemp  GE, White  HA, Pinneo  L, Addy  RF, Fom  AL, Lassa fever. Epidemiological aspects of the 1970 epidemic, Jos, Nigeria. Trans R Soc Trop Med Hyg. 1972;66:4028. DOIPubMed
  2. Frame  JD, Baldwin  JM, Gocke  DJ, Troup  JM. Lassa fever, a new virus disease of man from West Africa. I. Clinical description and pathological findings. Am J Trop Med Hyg. 1970;19:6706.PubMed
  3. Monath  TP, Maher  M, Casals  J, Kissling  RE, Cacciapuoti  A. Lassa fever in the Eastern Province of Sierra Leone, 1970-1972. II. Clinical observations and virological studies on selected hospital cases. Am J Trop Med Hyg. 1974;23:11409.PubMed
  4. Monath  TP, Mertens  PE, Patton  R, Moser  CR, Baum  JJ, Pinneo  L, A hospital epidemic of Lassa fever in Zorzor, Liberia, March-April 1972. Am J Trop Med Hyg. 1973;22:7739.PubMed
  5. Holmes  GP, McCormick  JB, Trock  SC, Chase  RA, Lewis  SM, Mason  CA, Lassa fever in the United States. Investigation of a case and new guidelines for management. N Engl J Med. 1990;323:11203. DOIPubMed
  6. Mahdy  MS, Chiang  W, McLaughlin  B, Derksen  K, Truxton  BH, Neg  K. Lassa fever: the first confirmed case imported into Canada. Can Dis Wkly Rep. 1989;15:1938.PubMed
  7. Zweighaft  RM, Fraser  DW, Hattwick  MA, Winkler  WG, Jordan  WC, Alter  M, Lassa fever: response to an imported case. N Engl J Med. 1977;297:8037. DOIPubMed
  8. Hirabayashi  Y, Oka  S, Goto  H, Shimada  K, Kurata  T, Fisher-Hoch  SP, An imported case of Lassa fever with late appearance of polyserositis. J Infect Dis. 1988;158:8725.PubMed
  9. Lloyd  G, Barber  GN, Clegg  JC, Kelly  P. Identification of Lassa fever virus infection with recombinant nucleocapsid protein antigen [letter]. Lancet. 1989;2:1222. DOIPubMed
  10. Johnson  KM, Monath  TP. Imported Lassa fever: reexamining the algorithms. N Engl J Med. 1990;323:113941. DOIPubMed
  11. Bowen  MD, Peters  CJ, Nichol  ST. Phylogenetic analysis of the Arenaviridae: patterns of virus evolution and evidence for cospeciation between arenaviruses and their rodent hosts. Mol Phylogenet Evol. 1997;8:30116. DOIPubMed
  12. Jahrling  PB, Frame  JD, Smith  SB, Monson  MH. Endemic Lassa fever in Liberia. III. Characterization of Lassa virus isolates. Trans R Soc Trop Med Hyg. 1985;79:3749. DOIPubMed
  13. Ruo  SL, Mitchell  SW, Kiley  MP, Roumillat  LF, Fisher-Hoch  SP, McCormick  JB. Antigenic relatedness between arenaviruses defined at the epitope level by monoclonal antibodies. J Gen Virol. 1991;72:54955. DOIPubMed
  14. Jahrling  PB, Frame  JD, Rhoderick  JB, Monson  MH. Endemic Lassa fever in Liberia. IV. Selection of optimally effective plasma for treatment by passive immunization. Trans R Soc Trop Med Hyg. 1985;79:3804. DOIPubMed
  15. Auperin  DD, McCormick  JB. Nucleotide sequence of the Lassa virus (Josiah strain) S genome RNA and amino acid sequence comparison of the N and GPC proteins to other arenaviruses. Virology. 1989;168:4215. DOIPubMed
  16. Clegg  JC, Wilson  SM, Oram  JD. Nucleotide sequence of the S RNA of Lassa virus (Nigerian strain) and comparative analysis of arenavirus gene products. Virus Res. 1991;18:15164. DOIPubMed
  17. ter Meulen  J, Koulemou  K, Wittekindt  T, Windisch  K, Strigl  S, Conde  S, Detection of Lassa virus antinucleoprotein immunoglobulin G (IgG) and IgM antibodies by a simple recombinant immunoblot assay for field use. J Clin Microbiol. 1998;36:31438.PubMed
  18. Demby  AH, Chamberlain  J, Brown  DW, Clegg  CS. Early diagnosis of Lassa fever by reverse transcription-PCR. J Clin Microbiol. 1994;32:2898903.PubMed
  19. World Health Organization. Lassa fever, case imported to Germany. Wkly Epidemiol Rec. 2000;75:178.PubMed
  20. Hufert  FT, Ludke  W, Schmitz  H. Epitope mapping of the Lassa virus nucleoprotein using monoclonal anti-nucleocapsid antibodies. Arch Virol. 1989;106:20112. DOIPubMed
  21. Felsenstein  J. PHYLIP (Phylogeny Inference Package) Version 3.57c. [Online] Department of Genetics, University of Washington, Seattle. Available from: URL: [28 January 2000, last date accessed.]
  22. McCormick  JB, King  IJ, Webb  PA, Johnson  KM, O'Sullivan  R, Smith  ES, A case-control study of the clinical diagnosis and course of Lassa fever. J Infect Dis. 1987;155:44555.PubMed
  23. Buckley  SM, Casals  J. Lassa fever, a new virus disease of man from West Africa. 3. Isolation and characterization of the virus. Am J Trop Med Hyg. 1970;19:68091.PubMed
  24. Djavani  M, Lukashevich  IS, Sanchez  A, Nichol  ST, Salvato  MS. Completion of the Lassa fever virus sequence and identification of a RING finger open reading frame at the L RNA 5' end. Virology. 1997;235:4148. DOIPubMed
  25. Günther  S, Sommer  G, von Breunig  F, Iwanska  A, Kalinina  T, Sterneck  M, Amplification of full-length hepatitis B virus genomes from samples from patients with low levels of viremia: frequency and functional consequences of PCR-introduced mutations. J Clin Microbiol. 1998;36:5318.PubMed
  26. Roche Molecular Biochemicals. Biochemicals Catalog. Mannheim: Roche Diagnostics GmbH; 2000:p. 50.
  27. Francis  SJ, Southern  PJ. Deleted viral RNAs and lymphocytic choriomeningitis virus persistence in vitro. J Gen Virol. 1988;69:1893902. DOIPubMed
  28. Krasko  AG, Moshnikova  AB, Kozhich  AT, Tchikin  LD, Ivanov  VT, Vladyko  AS, Lassa virus glycoproteins: antigenic and immunogenic properties of synthetic peptides to GP1. Arch Virol. 1990;115:1337. DOIPubMed
  29. Weber  EL, Buchmeier  MJ. Fine mapping of a peptide sequence containing an antigenic site conserved among arenaviruses. Virology. 1988;164:308. DOIPubMed
  30. Buchmeier  MJ, Southern  PJ, Parekh  BS, Wooddell  MK, Oldstone  MB. Site-specific antibodies define a cleavage site conserved among arenavirus GP-C glycoproteins. J Virol. 1987;61:9825.PubMed
  31. Vladyko  AS, Bystrova  SI, Malakhova  IV, Kunitskaia  L, Lemeshko  NN, Krasko  AG, The localization and preliminary characteristics of antigenic sites (B epitopes) in the nucleocapsid protein of the Lassa virus. Vopr Virusol. 1993;38:304.PubMed
  32. ter Meulen  J, Badusche  M, Kuhnt  K, Doetze  A, Satoguina  J, Marti  T, Characterization of human CD4+ T-cell clones recognizing conserved and variable epitopes of the Lassa virus nucleoprotein. J Virol. 2000;74:218692. DOIPubMed
  33. Kozak  M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986;44:28392. DOIPubMed
  34. Evans  DM, Dunn  G, Minor  PD, Schild  GC, Cann  AJ, Stanway  G, Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature. 1985;314:54850. DOIPubMed
  35. Kobiler  D, Rice  CM, Brodie  C, Shahar  A, Dubuisson  J, Halevy  M, A single nucleotide change in the 5' noncoding region of Sindbis virus confers neurovirulence in rats. J Virol. 1999;73:104406.PubMed
  36. Spotts  DR, Reich  RM, Kalkhan  MA, Kinney  RM, Roehrig  JT. Resistance to alpha/beta interferons correlates with the epizootic and virulence potential of Venezuelan equine encephalitis viruses and is determined by the 5' noncoding region and glycoproteins. J Virol. 1998;72:1028691.PubMed
  37. Wilson  SM, Clegg  JC. Sequence analysis of the S RNA of the African arenavirus Mopeia: an unusual secondary structure feature in the intergenic region. Virology. 1991;180:54352. DOIPubMed
  38. Lukashevich  IS. Generation of reassortants between African arenaviruses. Virology. 1992;188:6005. DOIPubMed
  39. ter Meulen  J. Lassa fever: implications of T-cell immunity for vaccine development. J Biotechnol. 1999;73:20712. DOIPubMed
  40. Fisher-Hoch  SP, McCormick  JB, Auperin  D, Brown  BG, Castor  M, Perez  G, Protection of rhesus monkeys from fatal Lassa fever by vaccination with a recombinant vaccinia virus containing the Lassa virus glycoprotein gene. Proc Natl Acad Sci U S A. 1989;86:31721. DOIPubMed
  41. Clegg  JC, Lloyd  G. Vaccinia recombinant expressing Lassa-virus internal nucleocapsid protein protects guineapigs against Lassa fever. Lancet. 1987;2:1868. DOIPubMed
  42. Morrison  HG, Bauer  SP, Lange  JV, Esposito  JJ, McCormick  JB, Auperin  DD. Protection of guinea pigs from Lassa fever by vaccinia virus recombinants expressing the nucleoprotein or the envelope glycoproteins of Lassa virus. Virology. 1989;171:17988. DOIPubMed
  43. La Posta  VJ, Auperin  DD, Kamin-Lewis  R, Cole  GA. Cross-protection against lymphocytic choriomeningitis virus mediated by a CD4+ T-cell clone specific for an envelope glycoprotein epitope of Lassa virus. J Virol. 1993;67:3497506.PubMed

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