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Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay

Donna J. JohnsonComments to Author , Eileen N. Ostlund, Douglas D. Pedersen, and Beverly J. Schmitt
Author affiliations: Animal and Plant Health Inspection Service, U. S. Department of Agriculture, Ames, Iowa, USA

Main Article

Table

Virus isolation compared with plaque reduction virus neutralization, immunoglobulin M-capture enzyme-linked immunosorbent assay serologic results, or both for West Nile virus samples positive by reverse transcription-nested polymerase chain reaction

Results
RT-nPCR positive samples No. tested VI positive VI negative Seropositive Seronegative
Equine brain 13 10 3 13 0
Equine plasma 8a 1 7 0 8b
Avian brain 7 7 0 NAc NA

RT-nPCR = reverse transcription-nested polymerase chain reaction; VI = virus isolation; NA = not applicable.
aMultiple plasma samples collected from two experimentally challenged ponies were RT-nPCR-positive for West Nile virus between 3 and 7 days after inoculation.
bWest Nile virus antibodies were not detected in either pony until beyond the time the virus was detected by RT-nPCR.
cNo avian serum was available for testing.

Main Article

Page created: April 27, 2012
Page updated: April 27, 2012
Page reviewed: April 27, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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